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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17 May 2007 to 14 June 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study undertaken by GLP accredited laboratory to internationally accepted guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Phenylphosphonic acid, zinc salt.
IUPAC Name:
Phenylphosphonic acid, zinc salt.
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): Phenylphosphonic acid, zinc salt
- Substance type: Inorganic
- Physical state: Powder
- Analytical purity: > 99%
- Purity test date: no data
- Lot/batch No.: 060725
- Expiration date of the lot/batch: no data
- Stability under test conditions: Stable
- Storage condition of test material: Room temperature
- Other:

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Chemicals Evaluation and Research Institute, Chemicals Assessment Center, 4-25, Koraku 1-chome, Bunkyo-ku, Tokyo, Japan
- Laboratory culture: Yes
- Method of cultivation: After cessation of aeration of the sludge mixture, the sludge particles were allowed to settle for 30 minutes. 300 mL supernatant was removed and 300 mL synthetic sewage was added to the mixture and the container was aerated again. This procedure was repeated once every day and thus obtained sludge was cultivated. The pH of cultivated sludge was kept at pH 7.0 ± 1.0 and the temperature of cultivation was kept at 25 ± 2°C.
- Storage conditions: Ambient
- Storage length: no data
- Preparation of inoculum for exposure: The activated sludge cultivated under the conditions described above was diluted with basal culture medium to prepare a sludge concentration of 900 mg/L based on the concentration of suspended solid in Section 12.1.1. Ten (10) mL of this diluted solution was inoculated to each test vessels of (2), (3) and (4) in Section 12.2.1 to prepare a concentration of suspended solid of 30 mg/L.
- Concentration of sludge: 30 mg/l
- Water filtered: yes/no
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium:
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 25°C
- pH: 7.0 ± 1.0
- pH adjusted: yes
- Aeration of dilution water: yes
- Suspended solids concentration: 30 mg/l
- Continuous darkness: no

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:

STATISTICAL METHODS:
Reference substance
Reference substance:
aniline

Results and discussion

Preliminary study:
No data
Test performance:
No unusual observations noted. It was confirmed that there is no leakage in the closed system before the BOD measurement.
% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
7 d
Remarks on result:
other: BOD
Parameter:
% degradation (O2 consumption)
Value:
-1
Sampling time:
14 d
Remarks on result:
other: BOD
Parameter:
% degradation (O2 consumption)
Value:
-3
Sampling time:
21 d
Remarks on result:
other: BOD
Parameter:
% degradation (O2 consumption)
Value:
-4
Sampling time:
28 d
Remarks on result:
other: BOD

BOD5 / COD results

Results with reference substance:
The percentage biodegradation of the reference substance (aniline) determined from the BOD after 14 days was 80%.

Any other information on results incl. tables

The pH of the test solution after cultivation period was 7.45 in (water + test substance) and 6.99,6.99 and 7.01 in (sludge + test substance).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
It is concluded that the test substance is not biodegradable.

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