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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
02 November 2007 to 30 November 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study undertaken at GLP accredited laboratory to internationally recognised guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Phenylphosphonic acid, zinc salt
IUPAC Name:
Phenylphosphonic acid, zinc salt
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): Phenylphosphonic acid zinc salt
- Substance type: Inorganic
- Physical state: Powder
- Analytical purity: > 98%
- Purity test date: No data
- Lot/batch No.: 0Q2YD
- Expiration date of the lot/batch:
- Stability under test conditions: Stable
- Storage condition of test material: Room temperature.
Radiolabelling:
no

Sampling and analysis

Details on sampling:
- Sampling intervals/frequency for test organisms: 4 and 3 days alternately.
- Sampling intervals/frequency for test medium samples: 4 and 3 days alternately.
- Sample storage conditions before analysis:
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Source: Commercial fish farm
- Age at study initiation (mean and range, SD): < 1 year
- Length at study initiation (lenght definition, mean, range and SD): average 7.5 cm
- Weight at study initiation (mean and range, SD): average 5.4 g
- Weight at termination (mean and range, SD):
- Method of breeding:
- Health status: good
- Description of housing/holding area: The fish were reared for 32 days in a static water chamber. (with circulating water cleaning filtering system)
- Feeding during test
- Food type: The formula feed for carp [manufactured by Nosan Corporation: crude protein of above 39.0%, crude fat of above 3.0%] added with animal fat and oil for cultivated aquatic animals [Riken Feed Oil Q: manufactured by Riken Vitamin Co., Ltd.]
- Amount: 5% w/w
- Frequency: 5% (w/w) was fed daily at the amount corresponding to about 1 - 2% of the body weight of the test fish. The amount was divided into halves, and feeding was twice in a day.

ACCLIMATION
- Acclimation period: 32 days
- Acclimation conditions (same as test or not): After rearing, the fish demonstrating any abnormalities were removed and fish to be assigned to the study groups were transferred in acclimatization chamber. An external disinfection was carried out for four days(October 12, 2007~ October 16,2007)before start to acclimatization. (Name of disinfectant: Sodium chloride, Concentration: 5.0glL, Temperature: 25'C)] Subsequently,
they were reared for 17 days in a flow through system.
- Type and amount of food: as above
- Feeding frequency: as above
- Health during acclimation (any mortality observed): no data

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
28 d

Test conditions

Hardness:
72 mg/l
Test temperature:
~ 25 °C
pH:
7.49
Dissolved oxygen:
8.1 mg/l
TOC:
< 0.3 mg/l
Salinity:
< 0.01 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 litre tank
- Material, size, headspace, fill volume: glass
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): 600 ml / min
- Renewal rate of test solution (frequency/flow rate): 72 ml / h
- No. of organisms per vessel: 45
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS

- Particulate matter: < 1.0mg/l
- Metals:
- Pesticides:
- Chlorine: < 0.01 mg/l
- Alkalinity: 7.49
- Ca/mg ratio:
- Conductance:
- Holding medium different from test medium: yes
- Intervals of water quality measurement: 3/4 days
- Intervals of test medium replacement: flow-through

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 h/day
- Light intensity: no data

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Nominal and measured concentrations:
No data
Reference substance (positive control):
no
Details on estimation of bioconcentration:
BASIS FOR CALCULATION OF BCF
Bioconcentration factors (BCF) of test substance in test fish were calculated using equations. Results were rounded off to 2 digits. From the detection limit concentration of test substance in fish body, if exceeded following magnifications, BCF could be calculated. (In case that the fish weight is 20.0 g).

Level 1 concentration: 3.1 fold
Level 2 concentration: 31 fold






Results and discussion

Lipid contentopen allclose all
Lipid content:
3.4 %
Time point:
start of exposure
Lipid content:
3.1
Time point:
end of exposure
Bioaccumulation factoropen allclose all
Type:
BCF
Value:
5 dimensionless
Basis:
whole body w.w.
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:0.030 mg/l
Type:
BCF
Value:
< 48 dimensionless
Basis:
whole body w.w.
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:0.0030 mg/l
Details on kinetic parameters:
No data
Metabolites:
No data
Results with reference substance (positive control):
No positive control
Details on results:
- Mortality of test organisms: none
- Behavioural abnormalities: none
- Observations on body length and weight:
- Other biological observations:
- Organ specific bioaccumulation:
- Bound residues forming a plateau:
- Mortality and/or behavioural abnormalities of control:
- Loss of test substance during test period:
- Results with vehicle control:

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Bioconcentration factors in a steady state were;
Level 1 concentration (0.030 mg/l) 5.0 fold;
Level 2 concentration (0.0030 mg/l): Less than 48 fold

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