Reaction mass of (R)-cyano(3-phenoxyphenyl)methyl rel-(1R,3R)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate and (R)-cyano(3-phenoxyphenyl)methyl rel-(1S,3S)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Insignificant route of exposure.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Five male rats per dose group were exposed to cyhalothrin in corn oil by intraperitoneal (i.p.) injection. Animals were observed for signs of intoxication daily up to 14 days.

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of (R)-cyano(3-phenoxyphenyl)methyl rel-(1R,3R)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate and (R)-cyano(3-phenoxyphenyl)methyl rel-(1S,3S)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate

EC Number:

932-389-6

Molecular formula:

C23H19ClF3NO3

IUPAC Name:

Reaction mass of (R)-cyano(3-phenoxyphenyl)methyl rel-(1R,3R)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate and (R)-cyano(3-phenoxyphenyl)methyl rel-(1S,3S)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate

Test animals

Species:

rat

Strain:

other: Alderley Park SPF-derived albino

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Animal Breeding Unit, ICI Ltd., Pharmaceuticals Division, Alderley Park, Macclesfield, Cheshire, UK
- Weight at study initiation: 119-220 g
- Fasting period before study: 16-20 h
- Housing: in stainless steel cages with a maximum of 5 animals per cage
- Diet (e.g. ad libitum): BP PCD pellets ad libitum
- Water (e.g. ad libitum): tap water ad libitum
- Acclimation period: at least 6 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-23°C
- Humidity (%): 50-60%
- Air changes (per hr): 24
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

corn oil

Details on exposure:

Volume applied: 10 mL/kg bw

Doses:

50, 250, 500, 750, and 1000 mg/kg bw

No. of animals per sex per dose:

5 males per dose group were used.

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

> 250 - < 750 mg/kg bw

Based on:

test mat.

Mortality:

In the 50 and 250 mg/kg bw dose groups no animal died. One animal died following a dose of 500 mg/kg bw. In the 750 mg/kg bw dose group, 2 animals were found dead and the remainder was terminated in extremis by day 4. All animals died in the 1000 mg/kg bw dose group.

Clinical signs:

Clinical signs included incontinence, scouring, subdued behaviour, piloerection, damp fur, tip-toe gait, hyperactivity, hypersensitivity to noise, ungroomed appearance, and at higher concentrations lacrimation, dehydration, ataxia, upward curvature of the spine, slow righting reflex, laboured respiration, hypothermia, and convulsions.

Applicant's summary and conclusion

Conclusions:

The study is not reliable because insignificant route of exposure.

Executive summary:

Acute toxicity of cyhalothrin to rats was determined by intraperitoneal application to 5 male animals per dose group at 50, 250, 500, 750, and 1000 mg/kg bw. Mortality and clinical signs were monitored daily for a period of 2 weeks. GLP compliance was stated.

Mortality in the 50, 250, 500, 750, and 1000 mg/kg bw groups was 0, 0, 1, 5, and 5 of five animals, respectively.

The LD50 of cyhalothrin after i.p. administration to male rats was therefore estimated to be in the range 250-750 mg/kg bw.