Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well performed study but without pproper documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report Date:
1983

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: UBA test proposal "Letale Wirkung beim Zebrabärbling Brachydanio rerio" dated 1982-06-01
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test solutions

Details on test solutions:
For the preparation of the stock solution 1 g of the substance were pre-solved in 1 g ethanol

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
length: 30 +-5 mm

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
15 ° dH
Test temperature:
23 +- 2 °C
pH:
7.0 +- 0.2
Details on test conditions:
vessel : 8.4 l
test duration: 96 hours, no feeding during that time
aeration: 3 bubbles per second
10 animals per 3.5 l of water
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 is greater than 100 mg/L.
Executive summary:

The substance was tested for toxicity to Brachydanio rerio according to the procedure proposed by the German Umweltbundesamt with the title "Letale Wirkung beim Zebrabärbling Brachydanio rerio" dated 1982 -06 -01. In a limit test 100 mg/l were administered to ten fish. After 96 hours no letality was observed. The LC50 has therefore to be considered as geater than 100 mg/L