3-nitro-p-anisic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed guideline study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-nitro-p-anisic acid

EC Number:

201-906-0

EC Name:

3-nitro-p-anisic acid

Cas Number:

89-41-8

Molecular formula:

C8H7NO5

IUPAC Name:

4-methoxy-3-nitrobenzoic acid

Details on test material:

purity: 98.2 %

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

source: domestic waste water treatment plant Frankfurt-Sindlingen

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

ca. 20 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

ca. 10 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

3

Sampling time:

3 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

7

Sampling time:

7 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

10

Sampling time:

14 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

14

Sampling time:

20 d

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

31

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

78% degradation after 14 days
85% degradation after 20 days
94% degradation after 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The substance is not readily biodegradable

Executive summary:

The substance was tested for ready biodegradability according to OECD guideline 301 B (modified Sturm test). Based on the test results and the definition of "ready biodegradability" the substance has to be considered as not readily biodegradable.