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Dodeca (lithium, sodium)-2-({4-[4-(4-{bis[alkyl-(sulfonatoalkoxy)-4-({-sulfonato-[(substituted-phenyl)diazenyl]phenyl} diazenyl)anilino]-triazin-yl}-6-[alkyl-(sulfonatoalkoxy)-4-(sulfonato--[(substituted-phenyl)diazenyl]phenyl)diazenyl)anilino]piperazin-yl)-triazin-ylamino]-alkyl-5-(sulfonatoalkoxy)phenyl}diazenyl)-5-[(sulfonatophenyl)diazenyl]benzensulfonate
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22-24 December 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted following the GLP principles and according to the OECD and Japanese guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: "Alga growth inhibition test, Daphnia sp. acute immobilisation test, and fish acute test" Yakushokuhatsu No. 1121002, Heisei 15.11.13 Seikyoku No. 2, Kanpokihatsu No. 031121002, November 21, 2003; the latest revision November 20, 2006.
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): E-BW102
- Substance type: reddish-brown powder
- Physical state: solid
- Lot/batch No.: MB-1
- Storage condition of test material: room temperature, shaded, air-tight
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10,18, 32, 56, 100
- Sampling method: from the middle layer of one vessel at teh time of preparation and at the end of exposure
- Sample storage conditions before analysis: not reported
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test solutions were prepared just before exposure. First, 200 mg test substance was dissolved in 2L dilution water resulting in a concentration of 100 mg/L. this was diluted further with dilution water resulting in concentrations of 56, 32, 18 and 10 mg/L. All these solutions were made in a final volume of 500 ml.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name:Daphnia
- Strain: Daphnia magna
- Source: National Institute for Environmental Studies, Japan
- Age at study initiation (mean and range, SD): < 24 h old juvenile females
- Feeding during test: none
ACCLIMATION
- Acclimation period: 48 h (for the parental Daphnida that produced the juveniles)
- Acclimation conditions (same as test or not): yes
- Type and amount of food: unicellular green algae, Chlorella vulgaris
- Feeding frequency: 6 mg C/2 L/ day for 25 daphnids
- Health during acclimation (any mortality observed): < 20 % mortality in parental daphnids 2 weeks prior the start of the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 54 mg/L CaCO3
- Test temperature:
- 19.8 - 20.3
- pH:
- 7.7 - 7.9
- Dissolved oxygen:
- 8.4 - 8.8 mg/L
- Nominal and measured concentrations:
- Nominal: 10, 18, 32, 56, 100 mg/L
Measured: 8.64, 17.0, 29.5, 52.8, 96.4 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Type: closed
- Material, size, headspace, fill volume: glass, 100 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated Yokohama city tap water
- Total organic carbon: < 0.3 mg/L
- Particulate matter: < 1 mg/L
- Metals: Al 0.05 mg/L
- Pesticides: < 0.001 mg/L
- Chlorine: < 0.1 mg/L
- Alkalinity: 37 mg CaCO3/L pH 4.8
- Ca/mg ratio: 14/3.3
- Conductivity: 13 mS/m
- Culture medium different from test medium: no
- Intervals of water quality measurement: every 6 months
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/ 8 h darkness
- Light intensity: 800 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : median effect concentration for immobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: yes
- Test concentrations: 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: 10% immobility was recorded after 48 h exposure to 100 mg/L in the range finding test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- the measured concentrations were within 20% of nominal
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: not reported
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no precipitation, floating solids, suspended solids
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: yes
- EC50: 0.81 mg/L (95 C.I. 0.75-0.87 mg/L)
- Other: the sensitivity tests were performed at every 6 months. the above results were the most recent in regard to the date of the experiment start.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this experiment, E-BW 102 did not induce acute immobilisation in daphnids up to 100 mg/L (EC50 > 100 mg/L).
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