Dodeca (lithium, sodium)-2-({4-[4-(4-{bis[alkyl-(sulfonatoalkoxy)-4-({-sulfonato-[(substituted-phenyl)diazenyl]phenyl} diazenyl)anilino]-triazin-yl}-6-[alkyl-(sulfonatoalkoxy)-4-(sulfonato--[(substituted-phenyl)diazenyl]phenyl)diazenyl)anilino]piperazin-yl)-triazin-ylamino]-alkyl-5-(sulfonatoalkoxy)phenyl}diazenyl)-5-[(sulfonatophenyl)diazenyl]benzensulfonate

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 February 2011 – 04May 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study has been performed according to OECD and EC guidelines and in compliance with GLP principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

(2001)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

(Official Journal of the European Union no. L142, May 31, 2008)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): E-BW102
- Description: Reddish brown powder
- Batch: MB-1
- Test substance storage: At room temperature protected from light
- Stability under storage conditions: Stable
- Expiry date: 23 December 2015

Radiolabelling:

no

Study design

Test temperature:

35°C +/- 1°C

HPLC method

Details on study design: HPLC method:

Analytical conditions
Instrument: Alliance Separation Module 2695 (Waters, Milford, MA, USA)
Detector: Dual λ Absorbance Detector 2487 (Waters)
Column: Hypersil BDS-CN, 150 mm x 4.6 mm i.d., dp = 5 μm (Thermo Fisher Scientific, Waltham, MA, USA)
Column temperature: 35°C +/- 1°
Mobile phase: 55/45 (v/v) methanol/water
Flow: 1 ml/min
Injection volume: 10 μl
UV detection: 210 nm

E-BW102 is an organic salt. Therefore, the test was performed at neutral pH.

Results and discussion

Adsorption coefficient

Adsorption coefficient 1

Type:

Koc

Value:

< 18

Temp.:

35 °C

Remarks on result:

other: HPLC estimation method, soil reference

Adsorption coefficient 2

Type:

log Koc

Value:

< 1.3

Temp.:

35 °C

Remarks on result:

other: HPLC estimation method, soil reference

Adsorption coefficient 3

Type:

Koc

Value:

< 33

Temp.:

35 °C

Remarks on result:

other: HPLC estimation method, sewage sludge reference

Adsorption coefficient 4

Type:

log Koc

Value:

< 1.52

Temp.:

35 °C

Remarks on result:

other: HPLC estimation method, sewage sludge reference

Results: HPLC method

Details on results (HPLC method):

In the chromatogram of the test substance solution, one peak with a retention time of 1.15 minutes was observed. Under the same analytical conditions, the retention time of acetanilide (log Koc (soil) = 1.26, log Koc (sewage sludge) = 1.52) was 2.28 minutes. Hence, it was concluded that the log Koc(soil) of the test substance was < 1.3 (Koc < 18) whereas the log Koc (sewage sludge) was < 1.52 (Koc < 33).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The HPLC method using soil/sewage sludge-adsorption-reference data was applied for the determination of the adsorption coefficient (Koc) of E-BW102. The Koc and log Koc values (soil) of the test substance at neutral pH were < 18 and < 1.3, respectively. The values for Koc and log Koc (sewage sludge) at netural pH were < 33 and < 1.52, respectively.