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Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16-20 May 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The test has been performed under GLP principles and following OECD and Chinese guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: State Environmental Protection Administration of China. The guidelines for the testing of chemicals, 203 fish acute toxicity test. China Environmental Science Press, 2004
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: State Environmental Protection Administration of China. The guidelines for the test of chemicals (HJ/T 153-2004), Beijing, 2004
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): E-BW 102
- Substance type: reddish brown powder
- Physical state: solid
- Stability under test conditions: stable
- Storage condition of test material: room temperature, avoiding light

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: control and 108 mg/L
- Sampling method: not reported
- Sample storage conditions before analysis: not reported

Test solutions

Vehicle:
no
Details on test solutions:
0.323 g test substance was dissolved in test water, ultrasonicated for 5 min and then the volume adjusted to 3 L such as that the final concentration was 108 mg/L

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Strain: Brachydanio rerio
- Source: Kang Da breding farm
- Age at study initiation (mean and range, SD): not reported
- Length at study initiation (length definition, mean, range and SD): 22.99 mm - 26.71 mm
- Weight at study initiation (mean and range, SD): 0.144 g - 0.321 g
- Method of breeding: not reported
- Feeding during test no feeding


ACCLIMATION
- Acclimation period: 53 days before the start of the experiment, flow-through
- Acclimation conditions (same as test or not): yes
- Type and amount of food: not reported
- Feeding frequency: daily
- Health during acclimation (any mortality observed): mortality 0% during 7 days before the start

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
mortality and abnormality recorded at 24, 48, 72 and 96 h

Test conditions

Hardness:
131.97 mg/L (CaCO3)
Test temperature:
21.7 - 24.9 C
pH:
7.14 - 7.96
Dissolved oxygen:
61 - 102 mg/L
Nominal and measured concentrations:
Nominal: 108 mg/L
Measured: 106 mg/L (averaged)
Details on test conditions:
TEST SYSTEM
- Test vessel: not reported
- Material, size, headspace, fill volume: 3L
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.0 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water



OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14 h light/10 h darkness
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: only a control and 108 mg/L used
- Justification for using less concentrations than requested by guideline: no mortality observed in the limit test
- Range finding study: no, only a limit test
- Test concentrations: 0 and 108 mg/L
- Results used to determine the conditions for the definitive study: no mortality or abnormal behavior observed
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 108 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
measured concentration was within 20% of nominal
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities: none
- Observations on body length and weight: not reported
- Other biological observations: not reported
- Mortality of control: none
- Other adverse effects control: not reported
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported
- Effect concentrations exceeding solubility of substance in test medium: no
Results with reference substance (positive control):
- Results with reference substance valid?
- Mortality: yes
- LC50: at 24 h was 301 mg/L (95 C.I of 262 - 346 mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test there was no mortality observed in zebra fish at concentrations of 108 mg/L E-BW 102 (LC50 > 108 mg/L).

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