Thiophosphoryl trichloride

Repeated dose toxicity: oral

Administrative data

Endpoint:

sub-chronic toxicity: oral

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: No GLP and no guideline study. The study has methodological deficiencies and is insufficient reported.

Data source

Reference

Reference Type:

publication

Title:

Short-term test systems to assess thiophosphoryl chloride standards

Author:

Colosi-Esca D, Snca Z, Barbarino F, Surcel D, Papilian VV

Year:

1984

Bibliographic source:

J. Appl. Toxicol. 4, 230-235

Materials and methods

Principles of method if other than guideline:

Thiophosphoryl trichloride in vegetable oil was mixed with dried milk powder and administered in warm water and given to female rats each morning 6 days a week prior to replenishing food hoppers. Body weights were measured weekly, relative organ weights, clotting time, gross and histopathological changes, hematological parameters, blood biochemical parameters (results were shown as graphics).

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Thiophosphoryl trichloride

EC Number:

223-622-6

EC Name:

Thiophosphoryl trichloride

Cas Number:

3982-91-0

Molecular formula:

Cl3PS

IUPAC Name:

phosphorothioyl trichloride

Details on test material:

no details given

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

Body weight at the beginning : approximately 105 g
no further data given

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: vegetable oil mixed in dried milk powder administered in warm water

Details on oral exposure:

Given each morning on 6 days per week for 90 days prior to replenishing the food hoppers.

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

90 days

Frequency of treatment:

6 days/week

Doses / concentrations

Remarks:

Doses / Concentrations:
7.5 or 37.5 mg/kg bw
Basis:

No. of animals per sex per dose:

Groups of 20 female rats

Control animals:

yes, concurrent vehicle

Details on study design:

Thiophosphoryl trichloride in vegetable oil was mixed with dried milk powder and administered in warm water and given to female rats each morning 6 days a week prior to replenishing food hoppers. Body weights were measured weekly, relative organ weights, clotting time, gross and histopathological changes, hematological parameters, blood biochemical parameters. Post-exposure period: no

Positive control:

no

Examinations

Observations and examinations performed and frequency:

DETAILED CLINICAL OBSERVATIONS No data

BODY WEIGHT: Yes
- Time schedule for examinations: weekly

OPHTHALMOSCOPIC EXAMINATION: No data

HAEMATOLOGY: Yes
total blood hemoglobin, hematocrit, mean corpuscular hemoglobin comcentration, differential leucocyte counts,

CLINICAL CHEMISTRY: Yes
serum alkaline phosphatase, serum cholin esterase, serum glutamate-oxalate transaminase, serum glutamate-pyrovate transaminase, total serum protein, u.a.

URINALYSIS: No data

NEUROBEHAVIOURAL EXAMINATION: No data

Sacrifice and pathology:

GROSS PATHOLOGY: Yes
relative organ weights: liver, heartm spleen, kidney and adremals gross changes

HISTOPATHOLOGY: Yes
changes in the above organs , histoenzymical changes in liver and gastric mucosa

Other examinations:

no data

Statistics:

Student's t-test

Results and discussion

Results of examinations

Clinical signs:

no effects observed

Mortality:

no mortality observed

Body weight and weight changes:

effects observed, treatment-related

Food consumption and compound intake (if feeding study):

not examined

Food efficiency:

not examined

Water consumption and compound intake (if drinking water study):

not examined

Ophthalmological findings:

not examined

Haematological findings:

effects observed, treatment-related

Clinical biochemistry findings:

effects observed, treatment-related

Urinalysis findings:

not examined

Behaviour (functional findings):

not examined

Organ weight findings including organ / body weight ratios:

effects observed, treatment-related

Gross pathological findings:

no effects observed

Histopathological findings: non-neoplastic:

effects observed, treatment-related

Histopathological findings: neoplastic:

no effects observed

Details on results:

i

Effect levels

1

Dose descriptor:

NOAEL

Remarks on result:

not determinable

Remarks:

no NOAEL identified

2

Dose descriptor:

LOAEL

Effect level:

ca. 7.5 mg/kg bw/day (actual dose received)

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

Data were presented as graphics only:
Increased growth rate in both dose groups, no dose related effect on organ weights, increase of liver transaminase activity in serum, decreased acid secretion in gastric mucosa, in the high dose group slight liver
dystrophic histopathological changes.

Applicant's summary and conclusion

Executive summary:

Groups of 20 female Wistar rats were given 0, 7.5 and 37.5 mg/kg bw/day thiophosphoryl trichloride in vegetable oil which was mixed with dried milk powder and administered in warm water, each morning, 6 days a week for 90 days, prior to replenishing food hoppers. A NOAEL could not be determined based in inconsistant responses in relative organ weights and biochemical and histochemical results. The LOAEL is 7.5 mg/kg bw/day under the condition of this investigation (Colosi-Esca 1984).