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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian germ cell study: cytogenicity / chromosome aberration
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: No GLP and no guideline study. The study has methodological deficiencies and is insufficient reported.

Data source

Reference
Reference Type:
publication
Title:
Short-term test systems to assess thiophosphoryl chloride standards
Author:
Colosi-Esca D, Snca Z, Barbarino F, Surcel D, Papilian VV
Year:
1984
Bibliographic source:
J. Appl. Toxicol. 4, 230-235

Materials and methods

Principles of method if other than guideline:
In a 30 day subchronic feeding study, young male and female Wistar rats were dosed at 5 % of the LD50 value (resp 37.5 mg/kg bw) as milk emulsion. Cytogenetic effects in bone marrow were analysed using the method of Ford and Woollam (1963).
GLP compliance:
not specified
Type of assay:
chromosome aberration assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Thiophosphoryl trichloride
EC Number:
223-622-6
EC Name:
Thiophosphoryl trichloride
Cas Number:
3982-91-0
Molecular formula:
Cl3PS
IUPAC Name:
phosphorothioyl trichloride
Details on test material:
no further data

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
no further data

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
Milk emulsion
Details on exposure:
In a 30 day subchronic feeding study, young male and female Wistar rats were dosed at 5 % of the LD50 value (resp 37.5 mg/kg bw) as milk emulsion. Cytogenetic effects in bone marrow were analysed using the method of Ford and Woollam (1963).
Duration of treatment / exposure:
30 day feeding study
Frequency of treatment:
daily
Post exposure period:
no data
Doses / concentrations
Remarks:
Doses / Concentrations:
37.5 mg/kg bw/day
Basis:

No. of animals per sex per dose:
no data
Control animals:
yes, concurrent vehicle
Positive control(s):
no data

Examinations

Tissues and cell types examined:
Bone marrow
Details of tissue and slide preparation:
no details given
Evaluation criteria:
no information given
Statistics:
no data

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Toxicity:
not specified
Vehicle controls validity:
valid
Negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
There were no significant differences in the incidence of abnormalities in the test group compared with the controls.

Any other information on results incl. tables

Negative, no change in the incidence of chromosomal aberrations in the femoral bone marrow.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
Executive summary:

In a 30 day subchronic feeding study, young male and female Wistar rats were dosed at 5 % of the LD50 value (resp 37.5 mg/kg bw) as milk emulsion. Cytogenetic effects in bone marrow were analysed using the method of Ford and Woollam (1963). There were no significant differences in the incidence of abnormalities in the test group compared with the controls (Colosi-Esca 1984).