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Although it has been shown that the solubility of RED SN is less than 0.1 mg/L or not existing, a median value for the Red SN hydrosolubility of 2 mg/L has been used for the ecotoxity and biodegradation assays, which corresponds to the excess of either the cation or the anion. Indeed Basic Red 1 (or even Dowfax 2A1) is present at a concentration defined as 2 mg/L.