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Diss Factsheets
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EC number: 236-144-8 | CAS number: 13189-00-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://www.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity of cosmetics ingredients licensed by the European Communities
- Author:
- Gocke E, King M-T, Eckhardt K and Wild D
- Year:
- 1 981
- Bibliographic source:
- Mutat. Res. 90: 91-109
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- X-linked recessive lethal mutations were examined in Drosophila melanogaster upto F3 generations to evaluate the mutagenic potential of the test material.
- GLP compliance:
- not specified
- Type of assay:
- Drosophila SLRL assay
Test material
- Reference substance name:
- Zinc sulphate
- EC Number:
- 231-793-3
- EC Name:
- Zinc sulphate
- Cas Number:
- 7733-02-0
- IUPAC Name:
- zinc sulfate
- Details on test material:
- - Name of test material (as cited in study report): Zinc sulphate
- Other: Obtained from Merck Co., Darmstadt (Germany)
Constituent 1
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- other: Berlin K (wild-type) and Basc
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- No data
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- - Vehicle(s)/solvent(s) used: no data
- Details on exposure:
- DIET PREPARATION
- Mixing appropriate amounts with (Type of food): 5 % saccharose - Duration of treatment / exposure:
- 3 successive broods
- Frequency of treatment:
- No data
- Post exposure period:
- No data
Doses / concentrations
- Remarks:
- Doses / Concentrations:
5 mM
Basis:
no data
- No. of animals per sex per dose:
- no data
- Control animals:
- yes
- Positive control(s):
- Trenimon
Examinations
- Tissues and cell types examined:
- X-chromosomes
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: Dose close to LD50 was selected for the study
- Evaluation criteria:
- No data
- Statistics:
- Significance was calculated according to the Kastenbaum-Bowman tables.
Results and discussion
Test results
- Key result
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF DEFINITIVE STUDY
- Statistical evaluation: A significant (P < 0.01) increase of sex-linked recessive lethals was observed in the Basc strain in only one test, whereas non-significant results were observed in two repeats. It has been assumed that the single increased value was due to chance.
Any other information on results incl. tables
Table 1 : Results of the Basc test in Drosophila Melanogaster
Compound |
Expt. no. |
Conc. (mM) |
Sex-linked recessive lethals/chromosomes tested and percentage |
||
Brood 1 |
Brood 2 |
Brood 3 |
|||
Zinc sulphate |
- |
5 |
13/1206** (1.08) |
2/1217 (0.16) |
4/1200 (0.33) |
- |
5 |
2/1233 (0.16) |
2/1175 (0.17) |
8/1193 (0.67) |
|
- |
5 |
3/1185 (0.25) |
1/1215 (0.08) |
1/1023 (0.10) |
|
|
|
18/3624* (0.50) |
5/3607 (0.14) |
13/3416 (0.38) |
|
Controls (cumulated) |
6 |
-# |
19/7130 (0.27) |
8/5525 (0.14) |
19/4871 (0.39) |
Positive control (trenimon) |
1 |
0.005 |
35/682** (5.13) |
23/598** (3.85) |
14/584** (2.40) |
# Different solvents were used in separate controls, *P ≤0.05, **P ≤0.01.
Applicant's summary and conclusion
- Conclusions:
- Under the test conditions, a single increased value observed was due to chance and did not indicate genotoxicity of zinc sulfate.
- Executive summary:
A sex-linked recessive lethal test was conducted in Drosophila melanogaster to evaluate the mutagenic potential of zinc sulfate. Berlin K (wild-type) and Base strains were used for the study. 5mM test concentration (dose close to the LD50) was applied by the adult feeding method in 5% saccharose. Controls and positive control (trenimon) were also included. Appoximately 1200 X-chromosomes were tested in each of 3 successive broods (3-3-4 days). In repeat experiments, sometimes only single broods were tested. To confirm X-linked recessive lethal mutations, F2 progeny cultures with 2 or fewer wild-type males were routinely retested in the F3 generation. Mosaics were not counted. "Clusters" of 2 were included because their occurrence was compatible with statistical expectation of independent origin. Significance was calculated according to the Kastenbaum-Bowman tables. A significant (P < 0.01) increase of sex-linked recessive lethals was observed in the Basc strain in only one test, whereas non-significant results were observed in two repeats. Under the test conditions, a single increased value observed was due to chance and did not indicate genotoxicity of zinc sulfate (Gocke, 1981).
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