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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Sep. 20, 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Qualifier:
according to guideline
Guideline:
other: ETAD 103
GLP compliance:
no
Analytical monitoring:
no
Details on sampling:
no data
Vehicle:
no
Details on test solutions:
Inoculum: 3 L laboratory scale unit (OECD)
Test organisms (species):
activated sludge
Details on inoculum:
Determination of dry weight: 1.00 g
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Hardness:
no data
Test temperature:
20.6 - 22.1 °C
pH:
7.9 - 8.6
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
nominal: 100, 1000 and 10000 mg/L
Details on test conditions:
Nutrient medium: according to ISO 8192-1986, chapter 4.2
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
All the respiration rate inhibitions with test substance were 0 % at the concentrations of 100, 1000 and 10000 mg/L.
Results with reference substance (positive control):
Test concentration Respiration rate inhibition
1 mg/L 6 %
20 mg/L 62 %
Validity criteria fulfilled:
yes
Conclusions:
No inhibition effects were observed up to the maximum tested concentration of 10000 mg/L. Therefore, the NOEC was determined to be 10000 mg/L and the EC50 to be >10000 mg/L (nominal conc.), respectively.
Executive summary:

The test was performed to determine the respiration rate inhibition on microorganisms of the test substance. This test was performed according to the ISO standard. Three concentrations of 100, 1000 and 10000 mg/L were set at the test exposure of 3 hours. The result showed all the respiration rate inhibitions were 0 % at the three test concentrations. In conclusion, the EC50 was >10000 mg/L (nominal conc.).

Description of key information

The toxicity to microorganims was determined in an ISO standard study. No inhibition effects were observed up to the maximum tested concentration of 10000 mg/L. Therefore, the NOEC was determined to be 10000 mg/L and the EC50 to be >10000 mg/L (nominal conc.), respectively.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
10 000 mg/L

Additional information

The available microorganism test was performed according to the ISO standard. Three concentrations of 100, 1000 and 10000 mg/L were set at the test exposure of 3 hours. The result showed all the respiration rate inhibitions were 0 % at the three test concentrations.

the NOEC was determined to be 10000 mg/L and the EC50 to be >10000 mg/L (nominal conc.), respectively.