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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06.05-1994 - 03.06.1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Aquatic mix of microorganism (activated) taken from activated sewage sludge plant for dometic waste water (Wupperverband). The sample was taken on 05.05.1994 and was not pre-treated prior to use.
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Inocullum concentration - 30 mg dry mass/L
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
2 d
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
10 d
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
20 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Details on results:
To calculate the degradation rates it was assumed that no nitrogen oxidation took place.
No toxicity on bacteria was observed at the concentration tested (100 mg/L).
Key result
Parameter:
COD
Value:
1 633 other: mg O2/g (theoretical oxygen demand)
Results with reference substance:
Aniline as reference substance was almost fully degraded (93%) within 28 days.
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Under the conditions of the test (manometric respirometry test) the substance was not readily biodegradable.
Executive summary:

Under the conditions of the test (manometric respirometry test) the test substance was not degraded within 28 days and, therefore, is considered not readily biodegradable according to internationally recognised OECD criteria.

Description of key information


The substance was tested in three independent screening tests for biodegradability: Ready biodegradability (Manometric Respirometry Test: 0 % biodegradation), inherent biodegradability (Zahn-WellensTest: 9 % biodegradation) and anaerobic biodegradability (ECETOC TR28 test: 2 % biodegradation). As a result, it can be concluded that the substance is hardly biodegradable potentially persistent in the environment.


 


Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Three independant tests have been performed with the substance throughout 1994 to assess degradation in the environment:

  • A Manometric Respiratory Test for assessment of ready biodegradability showing a biodegradation rate of 0 %,
  • A Zahn Wellens test for assessment of inherent biodegradability showing a biodegradation rate of 9 %, and
  • An ECETOC TR28 test to assess biodegradation under anaerob condition showing a degradation rate of 2 %.

In conclusion, it can be stated that the substance is hardly biodegradable.