6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

5.3.80 - 19.3.80

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study. No analytical purity given.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

(adopted 1981)

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid

EC Number:

279-505-5

EC Name:

6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid

Cas Number:

80584-91-4

Molecular formula:

C21H36N6O6

IUPAC Name:

6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid

Details on test material:

- Physical state: White powder

Test animals

Species:

rat

Strain:

other: RAI f. (SPF) (Ra 25)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: imported from Basle
- Age at study initiation: 5-6 weeks
- Average weight at study initiation: 113 g (males), 102 g (females)
- Fasting before study begin: 18 hours
- Housing: single housing
- Diet: A commercial autoclavable pelleted diet (Labsure CRM rat and mouse nuts), ad libitum.
- Water: Water filtered at 0.45 micron, ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 ± 2 °C
- Photoperiod: 12 hrs dark / 12 hrs light

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

0.5%

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 25% (w/v)
- Amount of vehicle: 20 mL/kg bw

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Observations: deaths and clinical symptoms were recorded (at least once daily).
- Necropsy of survivors performed: yes (at the end of the observation period, surviving animals were killed by exsanguination under ether anaesthesia)

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No mortality and no clinical signs were observed.

Mortality:

No mortality was observed.

Clinical signs:

other: No clinical signs were observed.

Gross pathology:

No abnormalities were seen at terminal autopsy.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information

Conclusions:

The oral LD50 in rats was determined to be greater than 5000 mg/kg body weight.

Executive summary:

In an oral acute toxicity study pre-dating GLP, five rats per sex were dosed with 5000 mg/kg of the test article in 0.5% CMC by gavage. Following a 14 day observation period all animals were sacrificed and a gross necropsy was performed. No mortality and no clinical signs were observed. No abnormalities were seen at terminal autopsy. As a result, the oral LD50 in rats was determined to be greater than 5000 mg/kg body weight.