6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

EL

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Source: fish farmer Branislav Settey, SKLOCHEM-AGROEKOLAB, Zvolen
- Length at study initiation: 2.0 +- 1.0 cm
- Feeding during test: none

ACCLIMATION
- Acclimation period:
held in laboratory 12 days before test
held in the water of the quality used in the test for at least 7 days.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

21.0 - 22.0 °C

pH:

8.12 - 8.20

Dissolved oxygen:

75.46% - 83.72% of saturation

Nominal and measured concentrations:

3300 mg/L (nominal concentration, initial)
measured concentrations: max. -0.86% of nominal

Details on test conditions:

TEST SYSTEM
- Type: closed
- Materia: glass or other chemically inert material
- Aeration: not during test
- No. of organisms per concentration: 7
- Biomass loading rate: max. 1.0 g fish/L

HOLDING WATER:
- dechlorinated tap water (left for at least 48 h in water canisters): Chlorine: < 10 µg/L, 151 mg/L (CaCO3)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO 6341 test water
- Intervals of water quality measurement: oxygen and pH measured daily

OTHER TEST CONDITIONS
- Adjustment of pH: yes, 1 N NaOH to pH 8.2 to ensure solubility of the test substance.
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED:
mortality recorded after 24, 48, 72 and 96 hours

TEST CONCENTRATIONS
- 3300 mg/L
- Range finding study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 3 300 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

no mortality observed in 96 h

Results with reference substance (positive control):

- Mortality: 96h-EC50 = 117.09 mg/L (mean of 3 determinations)

Validity criteria fulfilled:

yes

Executive summary:

The short term toxicity of TC to the fish Poecilia reticulata was tested according to OECD guideline 203 at static test conditions. No mortality was observed within 96 h at the only tested concentration of 3300 mg/L. LC50 (96 h) > 3300 mg/L.

Description of key information

The short term toxicity of TC to the fish Poecilia reticulata was tested according to OECD guideline 203. LC 50 (96 h) > 3300 mg/L.

Additional information

The short term toxicity of TC to the fish Oncorhynchus mykiss was tested according to OECD guideline 203. No mortality was observed within 96 h at 1000 mg/L, the highest concentration tested. LC50 (96 h) > 1000 mg/L.

The short term toxicity of TC to the fish Danio rerio was tested according to OECD guideline 203. No mortality was observed within 96 h at 1000 mg/L, the highest concentration tested. LC50 (96 h) > 1000 mg/L.