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EC number: 303-152-9 | CAS number: 94158-79-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 October 1992 to 06 November 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to EU & OECD test guidance in compliance with GLP
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Test concentrations: 0/220/ 500 mg/L
- Vehicle:
- no
- Details on test solutions:
- Water for dilution was added to the test substance, the mixture was homogenized using an Ultra-Turrax and Ultrasonic Bath and poured into the chamber under stirring with a glass rod.
The test batches were present as dark red solutions. No segregation or formation of precipitates could be observed.
In order to determine the substance concentration, water samples (approx. 20 mL) were taken from the middle of the test chamber using a screw-neck glass bottle at study start, and after 48 and 96 hours. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Species: zebrafish (Brachydanio rerio) (HAMILTON-BUCHANAN)
Origin: Central Toxicology (Hoechst AG)
Date of hatching: December 15, 1991
Delivery date: May 14, 1992
The fish were kept for 14 days in water for dilution before the start of the study under the following conditions:
Temperature: 22 ± 1 °C
Oxygen content: > 80 % of the saturation value
Duration of light period: 12 hours daily
Density: < 1 g fish / L water
Feeding: twice daily ad libitum
Food: Tetra Min, Tetra Werke, Melle
Prior to study start the body length of 10 representative fish from each batch was measured. These animals were not used during the study.
Part of study Batch No. Date n Body length (cm)
Variation range Mean s
1 16/91/H15/L2 23-Oct-92 10 3.4 – 3.9 3.6 ± 0.21
2 1/92/H1/L1 06-Nov-93 10 2.6 – 3.2 3.0 ± 0.17
Total sample 20 2.6 – 3.9 3.3 ± 0.35 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No post exposure observation
- Hardness:
- No data
- Test temperature:
- 21.0 - 22.3 °C
- pH:
- 7.0 - 7.8
- Dissolved oxygen:
- 6.1 - 10.1 mg/L
- Nominal and measured concentrations:
- 0, 220 and 500 mg/L
- Details on test conditions:
- Water for dilution
Reconstituted water, composition according to ISO/DIS 7346/1 was used as water for dilution.
Preparation was carried out in a unit consisting of two Hostalen®- lined steel vessels with a capacity of 1700 liters each.
The dilution water was prepared as described and was aerated until oxygen saturation. The pH was measured before the use of each study batch. It was in a range of 8.3 - 8.5.
Test conditions
The study was conducted in a static system. The test chambers, which were calibrated to 10 liters, were made of glass (length 30 cm, width 22 cm, height 24 cm) and stood in a water bath made of Hostalit Z® with a Plexiglas viewing panel. The temperature of the water bath was regulated by a thermostat to 22 + 1 °C. The chambers were illuminated from above from 06.00 a.m. to 06.00 p.m. The light intensity directly over the chambers was approximately 700 lux.
The test chambers were not aerated during the course of the study.
After the test concentration had been prepared and water parameters recorded, 10 fish were assigned at random to each test and control chamber. The fish received feed for the entire study period. Inspection of the fish took place after 2 - 4, 24, 48, 72 and 96 hours and involved recording the mortality and visible alterations in appearance and behaviour. Dead fish were removed from the chambers. Fish were considered dead when there was a lack of opercular movement and no response to slight mechanil stimulus. The water parameters were measured and recorded at the same dates. - Reference substance (positive control):
- not required
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 220 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Details on results:
- Recording of symptoms was disturbed by the dark red discoloration of the test water. The 220 mg/L group showed no effect in comparison with the control. The fish of the 500 mg/L group showed changes in behaviour and swimming behaviour.
- Results with reference substance (positive control):
- Not applicable.
- Reported statistics and error estimates:
- No data
- Sublethal observations / clinical signs:
0 mg/L (Control groups)
Hours after study start
Hours -----------------------------------à
2-4
24
48
72
96
Lethality (absolute)
0/20
0/20
0/20
0/20
0/20
No changes in appearance and behaviour
a
a
a
a
a
220 mg/L
Hours after study start
Hours ----------------------------------à
2-4
24
48
72
96
Lethality (absolute)
0/10
0/10
0/10
0/10
0/10
No changes in appearance and behaviour
a
a
a
a
s
Recording of symptoms was disturbed
x
x
x
x
s
500 mg/L
Hours after study start
Hours ----------------------------------à
2-4
24
48
72
96
Lethality (absolute)
0/10
0/10
0/10
0/10
1/10
No changes in appearance and behaviour
a
a
a
a
a
Recording of symptoms was disturbed
x
x
x
x
x
s = some fish
a = all fish
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 96-hour acute toxicity study of Reaktiv-Rot F-67787, only one fish died at 500 mg/L . No mortality occurred in the 220 mg/L group. The LC50 after 48 and 96 hours therefore was > 500 mg/L.
- Executive summary:
The acute toxicity to fish with Reaktiv-Rot F-67787 was conducted according to EU-Guideline C.1. Acute Toxicity in Fish Directive 84/449/EEC and theOECD-Guideline for Testing of Chemicals, 203 Fish Acute Toxicity Test. Study conducted in compliance with GLP.
Testing of lethal effects of Reaktiv-Rot F-67787 for 96 hours in Zebra fish (Brachydanio rerio) according to the method, only one fish died at 500 mg/L . No mortality occurred in the 220 mg/L group. The LC50 after 48 and 96 hours therefore was > 500 mg/L.
The test batches were present as dark red solutions.
In order to determine the substance concentration, water samples were taken from the middle of the test chamber at study start, and after 48 and 96 hours. All mean values were above 80 % of the nominal concentration.
Reference
Description of key information
LC50 (96h) > 500 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Remarks:
- (96h)
- Effect concentration:
- 500 mg/L
Additional information
The acute toxicity to fish of Reaktiv-Rot F-67787 was conducted according to EU-Guideline C.1. Acute Toxicity in Fish Directive 84/449/EEC and the OECD-Guideline for Testing of Chemicals, 203 Fish Acute Toxicity Test. The study was conducted in compliance with GLP.
In order to determine the substance concentration, water samples were taken from the middle of the test chamber at study start, and after 48 and 96 hours. All mean values were above 80% of the nominal concentration.
Testing of lethal effects of Reaktiv-Rot F-67787 for 96 hours in Zebra fish (Brachydanio rerio) up to 500 mg/L, only one fish died at 500 mg/L . No mortality occurred in the 220 mg/L group. The LC50 after 48 and 96 hours therefore was > 500 mg/L.Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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