Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
31 August 1998 to 01 September 1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Details on sampling:
Test concentrations (mg/L): 250, 500, 1000
Vehicle:
yes
Details on test solutions:
Concentrations were determined by dulution of a stock solution with distilled water.
Test organisms (species):
activated sludge
Details on inoculum:
Type: activated sludge (mixed population of different microorganisms)
Origin: waste water plant treating predominantly domestic sewage (Frankfurt-Sindlingen)
Pre-treatment: none
pH of the suspension before application: 7.2
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h
Post exposure observation period:
No post exposure observation period
Hardness:
No data
Test temperature:
20 ± 2°C
pH:
7.0 - 7.4
Dissolved oxygen:
Start 51.0 - 57.0 mg O2/L x h
Salinity:
Not applicable.
Nominal and measured concentrations:
250, 500, 1000 mg/L (nominal)
Details on test conditions:
A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3 5-dichlorophenol is used as a reference substance

The study is carried out with nominal weights. The test concentrations are not confirmed by chemical analysis.

Test concentrations (mg/L): 250, 500, 1000

Type of application: direct weighing
Test temperature 20 ± 2°C
Incubation time: 3 hours with permanent aeration.
Reference substance (positive control):
yes
Remarks:
3 5-dichlorophenol
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
Purity of the reference substance 62.7 %.

The reference substance concentration result from the quantities used in the preparations and have not been confirmed by analytical methods.

Further details in table form below.

Measured values of test substance / control / reference substance

  

STUDY EVALUATION Reference substance: 3.5-Dichlorophenol

Test concentration [mg/l]

Activity [mg O2/L . h]

Inhibition [%]

 Average

Control 1

51.0

-

 

 54.7

    

Control 2

56.0

-

Control 3

57.0

-

 DCP 5  30.0  45.1  -
 DCP 20  18.0  67.1  -
 DCP 30  14.0  74.4  -
 1000 (A)  54.0  1.2     - 0:6
 1000 (B)  56.0  - 2.4
 500 (A)  52.0 4.9      8.5
 500 (B)  48.0  12.2
 250 (A)  56.0  - 2.4     - 2.4
 250 (B)  56.0  - 2.4

 

Validity criteria fulfilled:
yes
Conclusions:
Toxicity of Reaktiv Rot F-67787 to activated sludge: EC50 > 1000 mg/L
Executive summary:

The study was conducted in accordance with GLP to the method specified under Commission Directive88/302/EEC ;Official Journal of the ECL 133.Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209).

 

A prescribed quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3 5-dichlorophenol is used as a reference substance.

 

Toxicity of Reaktiv Rot F-67787 to activated sludge: EC50 > 1000 mg/L. The substance is not classified for effects on this basis.

Description of key information

Toxicity of Reaktiv Rot F-67787 to activated sludge: EC50 > 1000 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L
EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information

The study was conducted in accordance with GLP to the method specified under Commission Directive88/302/EEC ;Official Journal of the EC L 133.Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209).

A prescribed quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3 5-dichlorophenol is used as a reference substance.

Toxicity of Reaktiv Rot F-67787 to activated sludge: EC50 > 1000 mg/L.The substance is not classified for effects on this basis.