4,4'-[(6-chloro-1,3,5-triazine-2,4-diyl)diimino]bis[5-hydroxy-6-[[4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonic] acid, sodium salt

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31 August 1998 to 01 September 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

Test concentrations (mg/L): 250, 500, 1000

Vehicle:

yes

Details on test solutions:

Concentrations were determined by dulution of a stock solution with distilled water.

Test organisms (species):

activated sludge

Details on inoculum:

Type: activated sludge (mixed population of different microorganisms)
Origin: waste water plant treating predominantly domestic sewage (Frankfurt-Sindlingen)
Pre-treatment: none
pH of the suspension before application: 7.2

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Post exposure observation period:

No post exposure observation period

Hardness:

No data

Test temperature:

20 ± 2°C

pH:

7.0 - 7.4

Dissolved oxygen:

Start 51.0 - 57.0 mg O2/L x h

Salinity:

Not applicable.

Nominal and measured concentrations:

250, 500, 1000 mg/L (nominal)

Details on test conditions:

A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3 5-dichlorophenol is used as a reference substance

The study is carried out with nominal weights. The test concentrations are not confirmed by chemical analysis.

Test concentrations (mg/L): 250, 500, 1000

Type of application: direct weighing
Test temperature 20 ± 2°C
Incubation time: 3 hours with permanent aeration.

Reference substance (positive control):

yes

Remarks:

3 5-dichlorophenol

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

Purity of the reference substance 62.7 %.

The reference substance concentration result from the quantities used in the preparations and have not been confirmed by analytical methods.

Further details in table form below.

Measured values of test substance / control / reference substance

  

STUDY EVALUATION Reference substance: 3.5-Dichlorophenol

Test concentration [mg/l]	Activity [mg O2/L . h]	Inhibition [%]	Average
Control 1	51.0	-	54.7
Control 2	56.0	-
Control 3	57.0	-
DCP 5	30.0	45.1	-
DCP 20	18.0	67.1	-
DCP 30	14.0	74.4	-
1000 (A)	54.0	1.2	- 0:6
1000 (B)	56.0	- 2.4
500 (A)	52.0	4.9	8.5
500 (B)	48.0	12.2
250 (A)	56.0	- 2.4	- 2.4
250 (B)	56.0	- 2.4

 

Validity criteria fulfilled:

yes

Conclusions:

Toxicity of Reaktiv Rot F-67787 to activated sludge: EC50 > 1000 mg/L

Executive summary:

The study was conducted in accordance with GLP to the method specified under Commission Directive88/302/EEC ;Official Journal of the ECL 133.Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209).

 

A prescribed quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3 5-dichlorophenol is used as a reference substance.

 

Toxicity of Reaktiv Rot F-67787 to activated sludge: EC50 > 1000 mg/L. The substance is not classified for effects on this basis.

Description of key information

Toxicity of Reaktiv Rot F-67787 to activated sludge: EC50 > 1000 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

EC10 or NOEC for microorganisms:

1 000 mg/L

Additional information

The study was conducted in accordance with GLP to the method specified under Commission Directive88/302/EEC ;Official Journal of the EC L 133.Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209).

A prescribed quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3 5-dichlorophenol is used as a reference substance.

Toxicity of Reaktiv Rot F-67787 to activated sludge: EC50 > 1000 mg/L.The substance is not classified for effects on this basis.