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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: screening test, other
Remarks:
Zahn-Wellens-Test
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 February to 11 March 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Conducted to a recognised OECD Guideline and to GLP.
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.9 (Biodegradation: Zahn-Wellens Test)
Qualifier:
according to guideline
Guideline:
other: ISO 9888 (1991)
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Oxygen conditions:
anaerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Kläranlagen der Hoechst AG: Werk Hächst und Werk Kelsterbach im Verhältnis 1: 1 gemischt.
- Preparation of inoculum for exposure:
- Pretreatment: Vorbehandlung entsprechend EG Vorschrift (1.6.1.3).
- Concentration of sludge: 9.4 g dry substance/L. Damit lag die Einsatzmenge im Test bei 658 mg/l (Trockensubstanz) und im geforderten Bereich des 2,5- bis 4-fachen des eingesetzten Kohlenstoffgehaltes (berechnet gemäß Stammläsungsanalyse).
- Initial cell/biomass concentration: 70 ml Schlammsuspension mit 100 g NS/L
Duration of test (contact time):
28 d
Initial conc.:
202.7 mg/L
Based on:
DOC
Details on study design:
As per OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Stammlösung : 2 g/I
Herstellung der Stamrnlösung: 4 g/1800 ml eingewogen; von pH 6,2 auf 6,9 eingestellt; 5 h bei 22°C gerührt; anschließender pH-Wert 6,7. Als klare Lösung abgefüllt.
Analysendaten der Stamrnlösung : DOC 463,5 mg/I [C]
Einsatzkonzentration, als DOC : 202,7 mg/l [C]
Reference substance:
diethylene glycol
Remarks:
20 g/l in deionised water
Preliminary study:
Not assessed
Test performance:
The test is considered valid in that the reference substance achieved > 75% degradation in 14 days, as specified by the guideline.
Parameter:
% degradation (DOC removal)
Value:
< 20
Sampling time:
28 d
Details on results:
Ein DOC-Abbau von Reaktiv-Rot F-67787 FW innerhalb des Prüfzeitraumes von 28 Tagen fand nicht statt.
Eine Adsorption an der Biomasse nach 3 Stunden wurde nicht festgestellt.
Die Elimination (Abbau und Adsorption) erreichte 0 %.
Results with reference substance:
The reference substance achieved > 75% degradation in 14 days.
Validity criteria fulfilled:
yes
Interpretation of results:
not inherently biodegradable
Conclusions:
The test substance is deemed not to be inherently biodegradable.
Executive summary:

The study was conducted via OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test). The results of the study indicated that the substance is not inherently biodegradable.

Endpoint:
biodegradation in water: screening test, other
Remarks:
COD measurement
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted to EU test guidance in compliance with GLP.
Qualifier:
according to guideline
Guideline:
EU Method C.6 (Degradation: Chemical Oxygen Demand)
Qualifier:
according to guideline
Guideline:
other: DIN 38 409 part 41
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
None
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Stammlösung
Einwaage der Prüfsubstanz: 1000 mg/L
pH-Wert nach Ansatz: 4.6
Der pH wurde nicht korrigiert.
Der Lösungsansatz wurde über 1 Stunde bei 21°C gerührt. Die Einwaage war unter den genannten Bedingungen vollständig löslich.
Reference substance:
other: Kaliumhydrogenphtalat
Preliminary study:
Not conducted.
Test performance:
The test was performed in accordance with the parameters specified to meet the requirements.
Details on results:
CSB-Bestimmungen der 1 : 10 verdünnten Stammlösung
1. Messung . . .. 66.0992
2. Messung . . . . 64.2114
3. Messung .... 68.7012

Mittelwert x Verdünnungsfaktor: 663 mg[O2]/L
Die Stammlösung enthielt 1 g/l der Prüfsubstanz.

Daraus ergibt sich ein CSB-Wert der Prüfsubstanz von 633 mg[O2]/g
Results with reference substance:
Referenzsubstanz : Kaliumhydrogenphtalat
Starnmlösung . . . . . . . . . . . . . .. : 170 mg/L in deionisiertem Wasser
berechneter CSB . . . . . . . . . . . .. : 200 mg/L (O2)
Validity criteria fulfilled:
yes
Conclusions:
Chemical oxygen demand: 633 mg[O2]/g

Description of key information

Not readily or inherently biodegradable

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information