Nicotinonitrile

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: According or similar to a guideline study, with acceptable restrictions. No data is provided on substance purity.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: not stated

Principles of method if other than guideline:

Test material was applied to the shaved and abraded skin of rabbits and covered with an occlusive dressing for 24 hours. The material was removed, skin gently rinsed and the animals were observed for 14 days for morbidity and mortality.

GLP compliance:

not specified

Remarks:

precedes establishment of guideline

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Nicotinonitrile

EC Number:

202-863-0

EC Name:

Nicotinonitrile

Cas Number:

100-54-9

Molecular formula:

C6H4N2

IUPAC Name:

pyridine-3-carbonitrile

Details on test material:

no data

Test animals

Species:

rabbit

Strain:

other: Albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

Four groups of four albino rabbits (2 male & 2 female) weighing between 2.0 and 3.0 kg each were employed in this study. All animals had their backs clipped free of hair 24 hours prior to testing. All of the animals had their backs abraded prior to dosing.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

All rabbits were weighed and the correct amount of experiemental material was applied to the back of each animal.

Doses:

0.5 gm/kg, 1.0 gm/kg, 2.0 gm/kg, 4.0 gm/kg

No. of animals per sex per dose:

4 groups of 4 albino rabbits (2 male and 2 female)

Details on study design:

14 day observation period

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

2 other: gm/kg

Based on:

test mat.

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

2 - 4 other: gm/kg

Based on:

test mat.

Mortality:

One male animal died at 2.0 gm/kg. Deaths occurred between 18-36 hours for males and females (2 each) at 4.0 gm/kg

Clinical signs:

other: No untoward symptoms were noted at 0.5 gm/kg. At 1.0 gm/kg, the animals were depressed after 24 hours, but appeared normal by 48 hours. At 2.0 gm/kg, the animals were severely depressed, ataxic, cold and demonstrating involuntary muscle tremors by 24 hour

Gross pathology:

Revealed nothing remarkable

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information LD50 of male/female is > 2000 mg/kg bw. Criteria used for interpretation of results: EU

Conclusions:

3-Cyanopyridine as tested in male rabbits has an acute dermal LD50 of approximately 2.0 gm/kg and an acute dermal LD50 of approximately 2.0 gm/kg - 4.0 gm/kg for female rabbits.