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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Reliable with restrictions; comparable to guideline study with acceptable restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1973

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: no information on method
Principles of method if other than guideline:
no information on method
GLP compliance:
no
Test type:
other: LD50
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Purity of Nicotinonitrile: not stated

Test animals

Species:
rat
Strain:
other: Sherman-Wistar
Sex:
male
Details on test animals and environmental conditions:
Rats weighed 200-300g

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
Rats were deprived of food, but not water, for 24 hours prior to dosing. Food and water were available ad libitum post dose.
Doses:
Concentrations of 250, 500, 1000, 2000 and 4000 mg/kg.
No. of animals per sex per dose:
Five male rats per group were dosed at 50% w/v suspension in water

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
1 100 mg/kg bw
Based on:
test mat.
95% CL:
960 - 1 500
Mortality:
250 mg/kg = 0/5
500 mg/kg = 0/5
1000 mg/kg = 2/5 — within 24 hours, animals were semi-comatose, which prevaled for five days during which time two animals died, one at three days post dose and the other four days post dose.
2000 mg/kg = 5/5
4000 mg/kg = 5/5
Clinical signs:
No signs of toxicity were observed at the 250 mg/kg dose level. At 500 mg/kg, the rats were depressed one hour post dose and lethargic. They appeared normal 24 hours post dose.
Body weight:
Rats in the 250 and 500 mg/kg dose levels gained weight during the 14-day observation period. A decrease in body weight for surviving animals was observed at study termination.
Gross pathology:
Findings revealed nothing remarkable.
Other findings:
Rats in the 1000 mg/kg dose group were extremely depressed 30 minutes post dose and exhibited shallow breathing. Within 24 hours they were semi-comatose. The surviving rats appeared normal eight to nine days post dose. At 2000 mg/kg and 4000 mg/kg the animals died within 1-3 hours.

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category IV
Remarks:
Migrated information LD50 > 300 mg/kg and < 2000 mg/kg Criteria used for interpretation of results: EU
Conclusions:
The acute oral LD50 of 3-cyanopyridine has been adequately characterized at 1100 mg/kg (95% Confidence limits = 960-1500 mg/kg)