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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EPA OPP 81-5 (Acute Dermal Irritation)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Divanadyl pyrophosphate
EC Number:
407-130-0
EC Name:
Divanadyl pyrophosphate
Cas Number:
65232-89-5
Molecular formula:
(VO)2P2O7
IUPAC Name:
Divanadyl pyrophosphate
Details on test material:
- Name of test material (as cited in study report): E-326 Catalyst
- Physical state: greyish-green powder
- Analytical purity: 100%
- Lot/batch No.: 3883559 A
- Storage condition of test material: room temperature

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Hazleton Research Animals Inc., Denver, Pennsylvania.
- Age at study initiation: young adults (at least 8 weeks old)
- Weight at study initiation: no data
- Housing: individually
- Diet: Lab Rabbit Chow HF (Purina #5326), 125 g/day while on test.
- Water: tap water, ad libitum.
- Acclimation period: 55 days.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16 - 21 °C
- Humidity (%): 30 - 70 %
- Photoperiod (hrs dark / hrs light): 12 h / 12 h

Test system

Type of coverage:
semiocclusive
Preparation of test site:
other: closely clipped
Vehicle:
physiological saline
Controls:
other: untreated skin of the same animals served as control
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 grams per site; two intact sites per animal
- % coverage: at least 10 % of the body area

VEHICLE
- Amount(s) applied (volume or weight with unit): 0.25 ml
Duration of treatment / exposure:
4 h
Observation period:
3 days
Number of animals:
6 (2 males, 4 females).
Details on study design:
TEST SITE
- Type of wrap if used: gauze and porous tape

REMOVAL OF TEST SUBSTANCE
- Washing (if done): excess test material was removed using dry gauze.
- Time after start of exposure: 4 h

SCORING SYSTEM: draize score

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 24 h - 48 - 72 h
Score:
0.1
Max. score:
4
Reversibility:
fully reversible within: 72 h
Irritation parameter:
edema score
Basis:
mean
Time point:
other: 24 h - 48 h - 72 h
Score:
0
Max. score:
4
Reversibility:
other: no effects

Any other information on results incl. tables

Erythema findings: right front/left front:

 Time  animal 1  animal 2  animal 3  animal 4  animal 5 animal 6
 0,5 h  1/0 0/1   0/0  0/1  0/0  0/0
 24 h  0/1  0/0  0/0  0/1  0/0  0/0
 48 h  1/1  0/0  0/0  0/1  0/0  0/0
 72 h  0/0  0/0  0/0  0/0  0/0  0/0

Edema findings: right front/left front:

 Time  animal 1  animal 2  animal 3  animal 4  animal 5 animal 6
 0,5 h  0/0   0/0  0/0    0/0   0/0   0/0
 24 h   0/0   0/0   0/0   0/0   0/0  0/0
 48 h   0/0   0/0   0/0   0/0  0/0   0/0
 72 h   0/0   0/0   0/0  0/0   0/0   0/0

Applicant's summary and conclusion

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