Vanadate(1-), oxo[phosphato(3-)-.kappa.O]-, hydrogen, hydrate (2:2:1)

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 81-5 (Acute Dermal Irritation)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Divanadyl pyrophosphate

EC Number:

407-130-0

EC Name:

Divanadyl pyrophosphate

Cas Number:

65232-89-5

Molecular formula:

(VO)2P2O7

IUPAC Name:

Divanadyl pyrophosphate

Details on test material:

- Name of test material (as cited in study report): E-326 Catalyst
- Physical state: greyish-green powder
- Analytical purity: 100%
- Lot/batch No.: 3883559 A
- Storage condition of test material: room temperature

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Hazleton Research Animals Inc., Denver, Pennsylvania.
- Age at study initiation: young adults (at least 8 weeks old)
- Weight at study initiation: no data
- Housing: individually
- Diet: Lab Rabbit Chow HF (Purina #5326), 125 g/day while on test.
- Water: tap water, ad libitum.
- Acclimation period: 55 days.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16 - 21 °C
- Humidity (%): 30 - 70 %
- Photoperiod (hrs dark / hrs light): 12 h / 12 h

Test system

Type of coverage:

semiocclusive

Preparation of test site:

other: closely clipped

Vehicle:

physiological saline

Controls:

other: untreated skin of the same animals served as control

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 grams per site; two intact sites per animal
- % coverage: at least 10 % of the body area

VEHICLE
- Amount(s) applied (volume or weight with unit): 0.25 ml

Duration of treatment / exposure:

4 h

Observation period:

3 days

Number of animals:

6 (2 males, 4 females).

Details on study design:

TEST SITE
- Type of wrap if used: gauze and porous tape

REMOVAL OF TEST SUBSTANCE
- Washing (if done): excess test material was removed using dry gauze.
- Time after start of exposure: 4 h

SCORING SYSTEM: draize score

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 h - 48 - 72 h

Score:

0.1

Max. score:

4

Reversibility:

fully reversible within: 72 h

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 h - 48 h - 72 h

Score:

0

Max. score:

4

Reversibility:

other: no effects

Any other information on results incl. tables

Erythema findings: right front/left front:

Time	animal 1	animal 2	animal 3	animal 4	animal 5	animal 6
0,5 h	1/0	0/1	0/0	0/1	0/0	0/0
24 h	0/1	0/0	0/0	0/1	0/0	0/0
48 h	1/1	0/0	0/0	0/1	0/0	0/0
72 h	0/0	0/0	0/0	0/0	0/0	0/0

Edema findings: right front/left front:

Time	animal 1	animal 2	animal 3	animal 4	animal 5	animal 6
0,5 h	0/0	0/0	0/0	0/0	0/0	0/0
24 h	0/0	0/0	0/0	0/0	0/0	0/0
48 h	0/0	0/0	0/0	0/0	0/0	0/0
72 h	0/0	0/0	0/0	0/0	0/0	0/0