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Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
Samples were collected of the Stock Solution (WAF) and of each treatment at the beginning and end of exposure for analysis.
Vehicle:
no
Details on test solutions:
Algal medium was prepared in accordance with OECD 201. Specifically, the AAP medium specified in Annex 3 from that method was used. Algae medium was prepared using deionized water (DI). Each of the stock solutions was prepared in DI . One ml of each stock was added to a flask and diluted to one liter with DI. This water was mixed on a magnetic stir plate and filtered through a 0.2 micron filter for sanitization. This medium was used for all culturing and testing of algae for this study.

Component Concentration in Prepared Medium mg/L
NaHCO3 15.0
NaNO3 25.5
MgCl2-6(H2O) 12.16
CaCl-2(H2O) 4.41
MgSO4-7H2O 14.6
K2HPO4 1.044
FeCl3-6(H2O) 0.160
Na2EDTA-2(H2O) 0.300
H3BO3 0.186
MnCl2-4(H2O) 0.415
ZnCl2 0.00327
CoCl2-6(H2O) 0.00143
Na2MoO4-(H2O) 0.00726
CuCl2-2(H2O) 0.000012
Test organisms (species):
other: Raphidocelis subcapitata
Details on test organisms:
An algal starter culture was purchased from Aquatic Biosystems, Inc. (Fort Collins,
Colorado) prior to testing. BMI cultures were inoculated at 104 cells/ml and allowed to
incubate for 72 hours. Cell counts were performed to ensure logarithmic growth. Testing
was performed using these cultures.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Nominal and measured concentrations:
Summary of Measured Concentrations
Nominal Concentration mg/L 0h mg/L 72h mg/L Concentration used for Calculations mg/L
0 BDL BDL 0
0.3125 BDL BDL 0.012
0.625 BDL BDL 0.023
1.25 BDL BDL 0.046
2.5 BDL BDL 0.092
5 BDL BDL 0.185
10 BDL BDL 0.369
Stock Solution 1 g/L (WAF) 36.9
Details on test conditions:
Test Requirement : Condition
1. Test type: Static
2. Renewal of test solutions: Not Renewed
3. Temperature (°C): 22 + 2°C
4. Light quality: Full Spectrum
5. Light intensity: 60 - 100 μE/(m-2 s-1)
6. Photoperiod: Continuous
7. Size of test vessel: 250 ml
8. Volume of test solution: 100 ml
9. Cell Density at test initiation: 104 cells/ml
11. Number of replicates per concentration: 3
14. Aeration: None
15. Medium: See section 3.2
16. Test duration: 72 hours
17. Test concentrations: Five product concentrations and a control
18. Dilution series: 0.1 dilution series for range-finding test
0.5 dilution series for definitive test
19. Endpoint: Growth Inhibition
20. Sampling and sample: WAF stock solution
21. Test acceptability criterion: Biomass of control cultures increase by a factor of at
least 16. Section – by – section specific growth rate
coefficient of variation not more than 35%.
Coefficient of variation during the whole test period
not greater than 7% in the controls.
Reference substance (positive control):
yes
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.062 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.13 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
The results of the range-finding study suggested that the endpoint would fall between 1 and 10 mg/L (Nominal WAF). Therefore, nominal treatments for the definitive test were 0, 0.3125,
0.625, 1.25, 2.5, 5.0 and 10 mg/L WAF.

The EC10 for specific growth rate was 0.062 mg/L based on measured concentrations. The EC50 for specific growth rate was 0.130 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
Aquatic toxicity study following OECD 201 was performed with 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine. The EC10 for specific growth rate was determined to be 0.062 mg/L based on measured concentrations. The
EC50 for specific growth rate was determined to be 0.130 mg/L.
Executive summary:

Aquatic toxicity study following OECD 201 was performed with 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine. The EC10 for specific growth rate was determined to be  0.062 mg/L based on measured concentrations. The

EC50 for specific growth rate was determined to be 0.130 mg/L.

Description of key information

Aquatic toxicity study following OECD 201 was performed with 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine. The EC10 for specific growth rate was determined to be  0.062 mg/L based on measured concentrations. The EC50 for specific growth rate was determined to be 0.130 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
0.13 mg/L
EC10 or NOEC for freshwater algae:
0.062 mg/L

Additional information