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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
Samples were collected of the Stock Solution (WAF) and of each treatment at the beginning and end of exposure for analysis.
Vehicle:
no
Details on test solutions:
Blacksburg, Virginia municipal tap water was dechlorinated using activated carbon,
deionized using mixed bed deionization tanks to a resistivity of > 1 megohm-cm, and
polished with a Millipore, Milli Q UV Plus and 0.2-micron filter to a final resistivity of ≥
18 megohm-cm. This water is referred to as BMI DI water.
This DI water was then chemically reconstituted according to U.S. EPA guidelines to a
final hardness of 160-180 (mg/l as CaCO3). This is BMI Hard Reconstituted Water
(HRW) and is the standard dilution water for testing with Daphnia magna.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna obtained from Aquatic Biosystems, Inc. (Fort Collins, Colorado) were
used for this study. Organisms were purchased and acclimated to BMI’s culture facility.
Once adults were produced, gravid females were segregated into fresh dilution water.
Organisms used in testing were < 24h old organisms from the 2rd to 5th generation. All
daphnid tests were initiated with offspring produced from BMI’s culture system and
obtained from a healthy stock culture showing no signs of stress such as >20% mortality,
presence of males or ephippiates, or discolored animals with no delay in production of
first brood.
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Details on test conditions:
Summary of Test Conditions for the Freshwater Daphnid Immobilisation Study
1. Test type: Static
2. Renewal of test solutions: Not Renewed
3. Temperature (°C): 25 + 1°C
4. Light quality: Ambient laboratory illumination
5. Light intensity: 50-100 foot candles (ambient laboratory
levels)
6. Photoperiod: 16 hr light/ 8 hr dark
7. Size of test vessel: 50 ml
8. Volume of test solution: 30 ml
9. Age of test organism: < 24 hour
10. Number of organisms per test vessel: 5
11. Number of replicates per concentration: 4
12. Total number of organisms per conc.: 20
13. Feeding regime: Not fed
14. Aeration: None
15. Dilution Water: HRW
16. Test duration: 48 hours
17. Test concentrations: Five product concentrations and a control
18. Dilution series: 0.1 dilution series for range-finding test
0.5 dilution series for definitive test
19. Endpoint: Immobilization (EC50)
20. Sampling and sample: 100 mg/L Water Accommodated Fraction
21. Test acceptability criterion: < 10% organism immobilization in controls,
≥ 2.0 mg/l dissolved oxygen
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
11.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Range Finding Results
The range finding test indicated that, in a definitive bioassay, there would likely be a result (EC50) between 100 and 1000 mg/L (nominal concentration).

The definitive test produced a 48-hour EC50 value of 11.4 mg/ based on the Spearman Karber statistical method. The 95% lower and upper confidence levels were 11.0 and 11.8 mg/L,
respectively.
Validity criteria fulfilled:
yes
Conclusions:
Daphnia magna was exposed to 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine in OECD 202 test. The definitive test produced a 48-hour EC50 value of 11.4 mg/ based on the Spearman Karber statistical method.
Executive summary:

Daphnia magna was exposed to 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine in OECD 202 test. The definitive test produced a 48-hour EC50 value of 11.4 mg/ based on the Spearman Karber statistical method.

Description of key information

Daphnia magna was exposed to 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine in OECD 202 test. The definitive test produced a 48-hour EC50 value of 11.4 mg/ based on the Spearman Karber statistical method.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
11.4 mg/L

Additional information