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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes (incl. certificate)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
The inoculum was prepared from a secondary effluent obtained from Peppers Ferry Wastewater Treatment Plant (Blacksburg, Virginia).
Duration of test (contact time):
28 d
Initial conc.:
30 mg/L
Based on:
test mat.
Initial conc.:
75 mg/L
Based on:
ThOD
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
A standard constant-pressure respirometer was used to measure oxygen consumption. There were seven flasks as follows: two flasks contained inoculum (“Blank”); two flasks contained inoculum and test material (“Test”); one flask contained a reference substance and inoculum (“Ref”); one flask contained inoculum with test material and the reference substance (“Tox”); one flask contained test material and a sterilizing agent (“Sterile”).

A small open container of soda lime pellets in each flask removed evolved CO2 from the gas phase. Each flask was gently stirred continuously using a magnetic stirrer. Volume-change readings were made at least daily in the testing apparatus. Re-establishment of equilibrium was performed as needed. Gas volume changes in test and reference flasks were compared to volume changes in the blanks to determine net oxygen consumption in the test chambers.

Test Environment
The test system was located in a secure area and was maintained at 22 ± 1 °C in near-darkness for the duration of the test.

Test Chronology
Testing proceeded as described in OECD 301F guidelines. Measurements of oxygen consumption were made daily. As oxygen consumption proceeded and/or atmospheric pressure changed, the cumulative volume change over those days was measured.

Re-establishment of equilibrium (reset) was performed at least daily, and more often when the extreme ranges of the graduated device were neared.
Reference substance:
acetic acid, sodium salt
Parameter:
% degradation (O2 consumption)
Value:
7.7
Sampling time:
10 d
Parameter:
% degradation (CO2 evolution)
Value:
7.9
Sampling time:
20 d
Parameter:
% degradation (CO2 evolution)
Value:
8.3
Sampling time:
28 d
Details on results:
The DEAPA/AEP Epoxy Resin Adduct 10-day window of degradation did not open within the study period. The product reached a maximum of 8.3% by test termination.
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine not reach pass levels for biodegradation within the 28-day period of the test, indicating that it is not readily biodegradable. The toxicity control treatment reached pass levels for biodegradation by day 7, indicating that the test substance is not inhibitory.
Executive summary:

The 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine not reach pass levels for biodegradation within the 28-day period of the test, indicating that it is not readily biodegradable. The toxicity control treatment reached pass levels for biodegradation by day 7, indicating that the test substance is not inhibitory.

Description of key information

The 4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 3-aminopropyldiethylamine and 2-piperazin-1-ylethylamine not reach pass levels for biodegradation within the 28-day period of the test, indicating that it is not readily biodegradable. The toxicity control treatment reached pass levels for biodegradation by day 7, indicating that the test substance is not inhibitory.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information