Monopentaerythritol tetraesters and dipentaerythritol hexaesters of 2-ethylhexanoic and n-valeric acids

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

Summary of available data used for the endpoint assessment of the target substance

Adequacy of study:

key study

Justification for type of information:

Please refer to the Analogue Justification provided in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

other:

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

source, CAS 647028-25-9, Croda, 1999, activated sludge, RL2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

source, CAS 647028-25-9, Croda, 1999, activated sludge, RL2

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

source, CAS 647028-25-9, Croda, 1999, activated sludge, RL2

Duration:

6 h

Dose descriptor:

other: EL10

Effect conc.:

> 50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

growth inhibition

Remarks on result:

other:

Remarks:

source, CAS 84418-63-3 (mono), Croda, 1993, activated sludge, P. putida growth inhibition, RL2

Duration:

6 h

Dose descriptor:

other: EL50

Effect conc.:

> 50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

growth inhibition

Remarks on result:

other:

Remarks:

source, CAS 84418-63-3 (mono), Croda, 1993, activated sludge, P. putida growth inhibition, RL2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

source, CAS 71010-76-9, Exxon, 2008, respiration inhibition, RL1

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

source, CAS 71010-76-9, Exxon, 2008, respiration inhibition, RL1

Description of key information

EL10 (6 h) > 50 mg/L nominal for Pseudomonas putida (ISO 10712); read-across.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

50 mg/L

Additional information

No study investigating the toxicity of Monopentaerythritol tetraesters and dipentaerythritol hexaesters of 2-ethylhexanoic and n-valeric acids to aquatic microorganisms is available. Therefore, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to the structurally related source substances Dipentaerythritol with fatty acids, C5 and C9 iso (CAS 647028-25-9), Isononanoic acid, mixed esters with dipentaerythritol, heptanoic acid and pentaerythritol (CAS 84418-63-3) and Decanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid (CAS 71010-76-9) is applied.

Based on the high degree of similarity between the structural and physico-chemical properties of the target and source substances, the source substances are considered suitable representatives for the evaluation of the toxicity of the target substance to aquatic microorganisms. The read-across approach is justified in detail within the analogue justification in IUCLID section 13.

The study with the source substance Dipentaerythritol with fatty acids, C5 and C9 iso (CAS 647028-25-9) investigated the toxicity to microorganisms in an activated sludge respiration inhibition test according to OECD guideline 209. Microorganisms were exposed to a limit concentration of 1000 mg/L (nominal) under static conditions. After 3 hours of exposure the study resulted in no effects on the respiration rate of an activated sludge microorganism population (EC50 (3 h): > 1000 mg/L; NOEC ≥ 1000 mg/L).

The study with the source substance Isononanoic acid, mixed esters with dipentaerythritol, heptanoic acid and pentaerythritol (CAS 84418-63-3) investigated the toxicity to Pseudomonas putida according to ISO 10712 under static conditions. Activated sludge was exposed for 6 hours to the water accommodated fraction (WAF) at nominal concentration (loading rate) of 50 mg/L. As no effects on growth inhibition occurred the EL10 and EL50 were determined to be > 50 mg/L.

The study with the source substance Decanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid (CAS 71010-76-9) investigated the toxicity to microorganism in an activated sludge respiration inhibition test according to OECD guideline 209. Microorganisms were exposed to nominal concentration of 10, 100, 1000 mg/L under static conditions. After 3 hours of exposure the study resulted in no effects on the respiration rate of an activated sludge microorganism population (EC50 (3 h): > 1000 mg/L; EC10 (3 h): > 1000 mg/L).

Based on the available results from structurally related source substances (in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5) which are characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that Monopentaerythritol tetraesters and dipentaerythritol hexaesters of 2-ethylhexanoic and n-valeric acids will not exhibit effects to activated sludge microorganisms and is not expected to inhibit the degradation process in sewage treatment plants.