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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
The study generally follows test guideline OECD203. The test report is without detailed documentation. The test parameters documented do not totally comply with the specific testing guideline, but are sufficient to accept the data.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
not specified
Specific details on test material used for the study:
Description: Dark Straw coloured liquid.
Storage: In original container at room temperature.
Analytical monitoring:
no
Vehicle:
no
Remarks:
Direct dispersion in water
Test organisms (species):
other: Salmo gairdneri
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 4.9 +/- 0.2 cm
- Weight at study initiation: 1.74 +/- 0.23 g



Test type:
semi-static
Water media type:
other: Dechlorinated tap water (Sodium thjosulphate).
Total exposure duration:
4 d
Hardness:
Aprox. 50 mg/l as CaCO3
Test temperature:
14 +/- 1°C
Nominal and measured concentrations:
Range finding study : 0, 0.1, 10 and 100 mg/l
Definitive study: 0, 10 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 l
- Aeration: yes
- Renewal rate of test solution: Daily
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.879 bodyweight/litre.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (Sodium thjosulphate).

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mortality (observation interval: 24h)

TEST CONCENTRATIONS
- Range finding study: 0, 0.1, 10 and 100 mg/l
- No of animals per concentration: 2:
- Results used to determine the conditions for the definitive study: 100% mortality after 24h at 100 mg/l, 0% mortality after 96 hat 10 mg/l
Reference substance (positive control):
no
Key result
Duration:
4 d
Dose descriptor:
LC50
Effect conc.:
> 10 - < 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities:
- Observations on body length and weight:
- Other biological observations:
- Mortality of control:
- Other adverse effects control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:
Definitive study: cumulative mortality (initial population: 10)
 Concentration (mg/l)  24 hours   48 hours   72 hours   96 hours
 Control  0
 10 (R1) 0
 10 (R2)  0

R1 - R2: replicates R1 - R2

Conclusions:
The 96h LC50 of the test material is >10 <=100 mg/l.

Description of key information

The 96h LC50  of the test material is >10 <=100 mg/l.

Key value for chemical safety assessment

Additional information