Reaction mass of N-butylphthalimide and N-sec-butylphthalimide and N-propylphthalimide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

The study generally follows test guideline OECD203. The test report is without detailed documentation. The test parameters documented do not totally comply with the specific testing guideline, but are sufficient to accept the data.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Specific details on test material used for the study:

Description: Dark Straw coloured liquid.
Storage: In original container at room temperature.

Analytical monitoring:

no

Vehicle:

no

Remarks:

Direct dispersion in water

Test organisms (species):

other: Salmo gairdneri

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 4.9 +/- 0.2 cm
- Weight at study initiation: 1.74 +/- 0.23 g

Test type:

semi-static

Water media type:

other: Dechlorinated tap water (Sodium thjosulphate).

Total exposure duration:

4 d

Hardness:

Aprox. 50 mg/l as CaCO3

Test temperature:

14 +/- 1°C

Nominal and measured concentrations:

Range finding study : 0, 0.1, 10 and 100 mg/l
Definitive study: 0, 10 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 l
- Aeration: yes
- Renewal rate of test solution: Daily
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.879 bodyweight/litre.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (Sodium thjosulphate).

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mortality (observation interval: 24h)

TEST CONCENTRATIONS
- Range finding study: 0, 0.1, 10 and 100 mg/l
- No of animals per concentration: 2:
- Results used to determine the conditions for the definitive study: 100% mortality after 24h at 100 mg/l, 0% mortality after 96 hat 10 mg/l

Reference substance (positive control):

no

Key result

Duration:

4 d

Dose descriptor:

LC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:
- Observations on body length and weight:
- Other biological observations:
- Mortality of control:
- Other adverse effects control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:

Sublethal observations / clinical signs:

Definitive study: cumulative mortality (initial population: 10)

Concentration (mg/l)	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
10 (R1)	0	0	0	0
10 (R2)	0	0	0	0

R1 - R2: replicates R1 - R2

Conclusions:

The 96h LC50 of the test material is >10 <=100 mg/l.

Description of key information

The 96h LC50  of the test material is >10 <=100 mg/l.