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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
for read across
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Related information:
Composition 1
Reason / purpose:
read-across source
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of invertebrate for the test chemical :1.To evaluate short term toxicity of test material on Biomphalaria alexandrina
GLP compliance:
not specified
Test material information:
Composition 1
Vehicle:
not specified
Details on test solutions:
1. Stock solution of test chemical was initially dissolved in dimethylsulfoxide (DMSO) to enhance the solubility, and serially diluted further with dechlorinated distilled water to achieve the desired series of concentrations. The final DMSO concentration in the experiments never exceeded 0.5% (v/v), and an equal amount was added to the control
Test organisms (species):
other: 1. Biomphalaria alexandrina 2. Daphnia magna
Details on test organisms:
1.- Common name: freshwater snail-8.072mm shell diameter- Source: The snails were collected from fresh water ponds (untreated with molluscicides) located at Bardalah village- Acclimation conditions (same as test or not): Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l ofdechlorinated tap water.- Food type: fed on dry lettuce leaves-oxygenated for 1 h daily, for 1 week prior to experimentation.-Number of organism : 62.- Source: D. magna were obtained from Dr. Freda Taub at the University of Washington-Age: 24 hr old-food : Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Remarks on exposure duration:
24 and 48 hr
Hardness:
1)136 mg/l (as CaCO3)2) 132 ppm total CaCO3
Test temperature:
1) 25 °C
pH:
1) 7.32) 7.2-7.5
Dissolved oxygen:
1) 6.8 mg/l.
Conductivity:
1) 352 µmΩ/cm
Details on test conditions:
1) - Common name: freshwater snail-8.072mm shell diameter- Source: The snails were collected from fresh water ponds (untreated with molluscicides) located at Bardalah village- Acclimation conditions (same as test or not): Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l ofdechlorinated tap water.- Food type: fed on dry lettuce leaves-oxygenated for 1 h daily, for 1 week prior to experimentation.-Number of organism : 62) - Test vessel: 250-mL glass beakers-Fill volume: 100 mL of the test solution- No. of vessels per control (replicates): 2- Photoperiod : light-dark cycle of 16:8 hr
Reference substance (positive control):
not specified
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
101.59 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
276 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
1.All data were subjected to analysis of variance (ANOVA) and means of LC50 values were compared for significance at the probability of 0.052.The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical (+)-Neomenthol is not likely to be toxic to fish atleast in the concentration range of 101.50 - 276.0 mg/l
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical (+)-Neomenthol (2216 -52 -6).The studies are as mentioned below:

1. The short term toxicity study of test material was evaluated for aquatic invertebrate Biomphalaria alexandrina snails .

 The snails were collected from fresh water ponds (untreated with molluscicides). Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l of dechlorinated tap water, fed on dry lettuce leaves and oxygenated for 1 h daily, for 1 week prior to experimentation. The median lethal effect concentration (LC50) for 24 h was observed to be 101.59 mg/l (with 95% confidence limits 89.45 – 115.4).

2. Short term toxicity of test material was evaluated for test material Ten neonates less than 24 hr old Daphnids were used for the test  . Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90.

 

Two replicates were used in each test. Daphnia were gently touched with a pastier pipet at 24 and 48 hr.If the daphnia could not swim actively for 15 s, immobilization (mortality) was recorded. The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis. The effect concentration of test chemical (EC50) for 50% immobilization of Daphnia magna was observed to be 276.0 mg/l.

On the basis of above effect concentration it can be considered that the test material has no toxic effect on aquatic invertebrates and cannot be classified as per CLP criteria.

 

Description of key information

Short term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical (+)-Neomenthol (2216 -52 -6).The studies are as mentioned below:

1. The short term toxicity study of test material was evaluated for aquatic invertebrate Biomphalaria alexandrina snails .

 The snails were collected from fresh water ponds (untreated with molluscicides). Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l of dechlorinated tap water, fed on dry lettuce leaves and oxygenated for 1 h daily, for 1 week prior to experimentation.The median lethal effect concentration (LC50) for 24 h was observed to be 101.59 mg/l (with 95% confidence limits 89.45 – 115.4).

2.Short term toxicity of test material was evaluated for test material Ten neonates less than 24 hr old Daphnids were used for the test  . Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90.

 Two replicates were used in each test. Daphnia were gently touched with a pastier pipet at 24 and 48 hr.If the daphnia could not swim actively for 15 s, immobilization (mortality) was recorded. The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis. The effect concentration of test chemical (EC50) for 50% immobilization of Daphnia magna was observed to be 276.0 mg/l.

On the basis of above effect concentration it can be considered that the test material has no toxic effect on aquatic invertebrates and cannot be classified as per CLP criteria.

 

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
276 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical (+)-Neomenthol (2216 -52 -6).The studies are as mentioned below:

1. The short term toxicity study of test material was evaluated for aquatic invertebrate Biomphalaria alexandrina snails .

 The snails were collected from fresh water ponds (untreated with molluscicides). Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l of dechlorinated tap water, fed on dry lettuce leaves and oxygenated for 1 h daily, for 1 week prior to experimentation.The median lethal effect concentration (LC50) for 24 h was observed to be 101.59 mg/l (with 95% confidence limits 89.45 – 115.4).

2.Short term toxicity of test material was evaluated for test material Ten neonates less than 24 hr old Daphnids were used for the test  . Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90.

 Two replicates were used in each test. Daphnia were gently touched with a pastier pipet at 24 and 48 hr.If the daphnia could not swim actively for 15 s, immobilization (mortality) was recorded. The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis. The effect concentration of test chemical (EC50) for 50% immobilization of Daphnia magna was observed to be 276.0 mg/l.

On the basis of above effect concentration it can be considered that the test material has no toxic effect on aquatic invertebrates and cannot be classified as per CLP criteria.