Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across: supporting information
Related information:
Composition 1
Reason / purpose:
read-across: supporting information
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two toxicity study of aquatic algae and cyanobacteria for the test chemical :1 and 2. Toxicity of test material of aquatic algae and cyanobacteria
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- IUPAC Name: (+)-Neomenthol- InChI: 1S/C10H20O/c1-7(2)9-5-4-8(3)6-10(9) 11/h7-11H,4-6H2,1-3H3/t8-,9+,10+/m1/s1- Smiles: C[C@@H]1CC[C@H]([C@H](C1)O)C(C)C- Name of test material :d-Neomenthol- Molecular formula:C10H20O- Molecular weight :156.267 g/mol- Substance type:Organic
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
1.Source: Dr. Freda Taub, University of Washington TSeattle, WA)Methode of culturing : Stock cultures of algae were maintained on 1.5% Difco-Bacto agar slants.initial cell density : 4.0 X 104 algal cells per milliliter
Test type:
static
Water media type:
freshwater
Total exposure duration:
7 d
Details on test conditions:
1.- Test vessel: 500 ml Erlenmeyer flasks with ground glass stoppers-fill volume : 100 ml-Test algae were grown in a semiflow-through culture apparatus on T82MV(7) and taken during log phase growth for inoculation into the test flasks.
Duration:
96 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
cell number
Duration:
7 d
Dose descriptor:
EC0
Effect conc.:
263 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
growth rate
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical (+)-Neomenthol is not likely to be toxic to fish atleast in the concentration range of 100-263 mg/l
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical (+)-Neomenthol (2216 -50 -6).The studies are as mentioned below:

1.Toxicity of test material on aquatic algae and cyanobacteria was evaluated on S. capricornutum.

Stock cultures of algae were maintained on 1.5% Difco-Bacto agar slants. Test algae were grown in a semiflow-through culture apparatus on T82MV(7) and taken during log phase growth for inoculation into the test flasks. 500 ml Erlenmeyer flasks with ground glass stoppers were used as test chambers. 100 ml of media was placed in each test chamber and inoculated with approximately 4.0 X 104 algal cells per milliliter. The algae were placed in an incubator . Using a Newbauer Counting Chamber, cell densities were determined every 24 hr for 5 consecutive days. The effect concentartion (IC50) after 96 h for the test material was observed to be >100 mg/l.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.

2. Toxicity of test material was evaluted for Scenedesmus subspicatus (Green Algae ) in a static cocndition for 7 days. The effect concentration (EC0) of test material on Scenedesmus subspicatus (Green Algae ) after 7 days was observed to be 263 mg/l. Based on the above test concentartion it can be considered that the test material is non toxic to aquatic algae and hence, can be considered to be not classified as per CLP classification.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.

Description of key information

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical (+)-Neomenthol (2216 -50 -6).The studies are as mentioned below:

1.Toxicity of test material on aquatic algae and cyanobacteria was evaluated on S. capricornutum.

Stock cultures of algae were maintained on 1.5% Difco-Bacto agar slants. Test algae were grown in a semiflow-through culture apparatus on T82MV(7) and taken during log phase growth for inoculation into the test flasks. 500 ml Erlenmeyer flasks with ground glass stoppers were used as test chambers. 100 ml of media was placed in each test chamber and inoculated with approximately 4.0 X 104 algal cells per milliliter. The algae were placed in an incubator . Using a Newbauer Counting Chamber, cell densities were determined every 24 hr for 5 consecutive days. The effect concentartion (IC50) after 96 h for the test material was observed to be >100 mg/l.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.

2. Toxicity of test material was evaluted for Scenedesmus subspicatus (Green Algae ) in a static cocndition for 7 days. The effect concentration (EC0) of test material on Scenedesmus subspicatus (Green Algae ) after 7 days was observed to be 263 mg/l. Based on the above test concentartion it can be considered that the test material is non toxic to aquatic algae and hence, can be considered to be not classified as per CLP classification.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.

Key value for chemical safety assessment

EC50/LC50 for freshwater algae:
100 mg/L

Additional information

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical (+)-Neomenthol (2216 -50 -6).The studies are as mentioned below:

1.Toxicity of test material on aquatic algae and cyanobacteria was evaluated on S. capricornutum.

Stock cultures of algae were maintained on 1.5% Difco-Bacto agar slants. Test algae were grown in a semiflow-through culture apparatus on T82MV(7) and taken during log phase growth for inoculation into the test flasks. 500 ml Erlenmeyer flasks with ground glass stoppers were used as test chambers. 100 ml of media was placed in each test chamber and inoculated with approximately 4.0 X 104 algal cells per milliliter. The algae were placed in an incubator . Using a Newbauer Counting Chamber, cell densities were determined every 24 hr for 5 consecutive days. The effect concentartion (IC50) after 96 h for the test material was observed to be >100 mg/l.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.

2. Toxicity of test material was evaluted for Scenedesmus subspicatus (Green Algae ) in a static cocndition for 7 days. The effect concentration (EC0) of test material on Scenedesmus subspicatus (Green Algae ) after 7 days was observed to be 263 mg/l. Based on the above test concentartion it can be considered that the test material is non toxic to aquatic algae and hence, can be considered to be not classified as per CLP classification.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.