(+)-neomenthol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

for read across

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of invertebrate for the test chemical :1.To evaluate short term toxicity of test material on Biomphalaria alexandrina

GLP compliance:

not specified

Vehicle:

not specified

Details on test solutions:

1. Stock solution of test chemical was initially dissolved in dimethylsulfoxide (DMSO) to enhance the solubility, and serially diluted further with dechlorinated distilled water to achieve the desired series of concentrations. The final DMSO concentration in the experiments never exceeded 0.5% (v/v), and an equal amount was added to the control

Test organisms (species):

other: 1. Biomphalaria alexandrina 2. Daphnia magna

Details on test organisms:

1.- Common name: freshwater snail-8.072mm shell diameter- Source: The snails were collected from fresh water ponds (untreated with molluscicides) located at Bardalah village- Acclimation conditions (same as test or not): Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l ofdechlorinated tap water.- Food type: fed on dry lettuce leaves-oxygenated for 1 h daily, for 1 week prior to experimentation.-Number of organism : 62.- Source: D. magna were obtained from Dr. Freda Taub at the University of Washington-Age: 24 hr old-food : Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

24 and 48 hr

Hardness:

1)136 mg/l (as CaCO3)2) 132 ppm total CaCO3

Test temperature:

1) 25 °C

pH:

1) 7.32) 7.2-7.5

Dissolved oxygen:

1) 6.8 mg/l.

Conductivity:

1) 352 µmΩ/cm

Details on test conditions:

1) - Common name: freshwater snail-8.072mm shell diameter- Source: The snails were collected from fresh water ponds (untreated with molluscicides) located at Bardalah village- Acclimation conditions (same as test or not): Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l ofdechlorinated tap water.- Food type: fed on dry lettuce leaves-oxygenated for 1 h daily, for 1 week prior to experimentation.-Number of organism : 62) - Test vessel: 250-mL glass beakers-Fill volume: 100 mL of the test solution- No. of vessels per control (replicates): 2- Photoperiod : light-dark cycle of 16:8 hr

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

101.59 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

276 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

1.All data were subjected to analysis of variance (ANOVA) and means of LC50 values were compared for significance at the probability of 0.052.The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical (+)-Neomenthol is not likely to be toxic to fish atleast in the concentration range of 101.50 - 276.0 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical (+)-Neomenthol (2216 -52 -6).The studies are as mentioned below:

1. The short term toxicity study of test material was evaluated for aquatic invertebrate Biomphalaria alexandrina snails .

 The snails were collected from fresh water ponds (untreated with molluscicides). Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l of dechlorinated tap water, fed on dry lettuce leaves and oxygenated for 1 h daily, for 1 week prior to experimentation. The median lethal effect concentration (LC50) for 24 h was observed to be 101.59 mg/l (with 95% confidence limits 89.45 – 115.4).

2. Short term toxicity of test material was evaluated for test material Ten neonates less than 24 hr old Daphnids were used for the test  . Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90.

 

Two replicates were used in each test. Daphnia were gently touched with a pastier pipet at 24 and 48 hr.If the daphnia could not swim actively for 15 s, immobilization (mortality) was recorded. The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis. The effect concentration of test chemical (EC50) for 50% immobilization of Daphnia magna was observed to be 276.0 mg/l.

On the basis of above effect concentration it can be considered that the test material has no toxic effect on aquatic invertebrates and cannot be classified as per CLP criteria.

 

Description of key information

Short term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical (+)-Neomenthol (2216 -52 -6).The studies are as mentioned below:

1. The short term toxicity study of test material was evaluated for aquatic invertebrate Biomphalaria alexandrina snails .

 The snails were collected from fresh water ponds (untreated with molluscicides). Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l of dechlorinated tap water, fed on dry lettuce leaves and oxygenated for 1 h daily, for 1 week prior to experimentation.The median lethal effect concentration (LC50) for 24 h was observed to be 101.59 mg/l (with 95% confidence limits 89.45 – 115.4).

2.Short term toxicity of test material was evaluated for test material Ten neonates less than 24 hr old Daphnids were used for the test  . Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90.

 Two replicates were used in each test. Daphnia were gently touched with a pastier pipet at 24 and 48 hr.If the daphnia could not swim actively for 15 s, immobilization (mortality) was recorded. The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis. The effect concentration of test chemical (EC50) for 50% immobilization of Daphnia magna was observed to be 276.0 mg/l.

On the basis of above effect concentration it can be considered that the test material has no toxic effect on aquatic invertebrates and cannot be classified as per CLP criteria.

 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

276 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical (+)-Neomenthol (2216 -52 -6).The studies are as mentioned below:

1. The short term toxicity study of test material was evaluated for aquatic invertebrate Biomphalaria alexandrina snails .

 The snails were collected from fresh water ponds (untreated with molluscicides). Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l of dechlorinated tap water, fed on dry lettuce leaves and oxygenated for 1 h daily, for 1 week prior to experimentation.The median lethal effect concentration (LC50) for 24 h was observed to be 101.59 mg/l (with 95% confidence limits 89.45 – 115.4).

2.Short term toxicity of test material was evaluated for test material Ten neonates less than 24 hr old Daphnids were used for the test  . Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90.

 Two replicates were used in each test. Daphnia were gently touched with a pastier pipet at 24 and 48 hr.If the daphnia could not swim actively for 15 s, immobilization (mortality) was recorded. The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis. The effect concentration of test chemical (EC50) for 50% immobilization of Daphnia magna was observed to be 276.0 mg/l.

On the basis of above effect concentration it can be considered that the test material has no toxic effect on aquatic invertebrates and cannot be classified as per CLP criteria.