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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Mar 5, 2015 to Jun 17, 2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
2008
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch No. 6F11027000
- Expiration date of the lot/batch: 2 years after first opening (17-Nov-2017)
- Purity: 96%
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A saturated solution was prepared be adding 117 µl/L test item (eq. to 100 mg/L) to corresponding amount of dilution water and stirring slowly for 23.5 hrs
- Eluate: The lower phase of the resulting solution
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain/variety: Berlin/Straus
- Sex: female
- Source: bred in-house at LAUS GmbH. Origin is Umweltbundesamt Berlin
- Food type: unicellular green algae (Demodesmus subspicatus)
- Medium: M4-Medium (husbandry) and ISO-Medium (test)
- Medium renewal: twice/week

SELECTION OF DAPHNIA
- ~20hrs before start of test, adult animals separated from the young
- 0.5 hrs before test start, newborn daphnia (aged between 0- 23hrs) were sieved from medium and immediately placed in 250-mL beaker containing dilution water
- after a settling-in period of 30 min, animals which showed no apparent damage were used for the test
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
250 mg (CaCO3)/L
Test temperature:
18.5-20.8 °C
Dissolved oxygen:
The lowest concentration of dissolved oxygen at the end of the test was 8.7 mg/L.
Nominal and measured concentrations:
100 mg/L,nominal (0.19 mg/L measured geometric mean)
Details on test conditions:
TEST SYSTEM
- Test vessel: glass flask, total volume 65 mL
- Type: closed
- Aeration: None
- No. of organisms per vessel: 5 daphnia
- No. of vessels per concentration: 4 vessels
- No. of vessels per control: 2 vessels

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water enriched with certain minerals
- Hardness: 250 mg (CaCO3)/L

OTHER TEST CONDITIONS
- Lighting: Diffuse lighting
- Feeding during test: None

EFFECT PARAMETERS MEASURED: Immobility; observed at 24 and 48 hrs
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.19 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.19 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 0.19 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
0.19 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The study was performed as a limit test at the nominal concentration of 100 mg/L. Twenty daphnia were exposed to the test item for 48 hours in a static test system. The concentration showed no significant toxicity (5 % immobilisation, respectively one animal). None of the animals were immobilised in the control.
Results with reference substance (positive control):
24-hr EC50 = 1.2 mg/L. Was within the demanded range of 0.6-1.7 mg/L

Validation Summary of Study

  • The 24h-EC50 of K2Cr2O7 should lie between 0.6 and 1.7mg/L: The 24h-EC50 of K2Cr2O7 was determined as 1.2 mg/L in a separate GLP study
  •  Immobilisation in the controls may not exceed 10 %: Immobilisation in the controls was 0%
  • The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L: The lowest concentration of dissolved oxygen at the end of the test was 8.7mg/L
  • The pH-value in the test solutions should not vary by more than 1.5 units during the test: The highest variation was 0.1 units
Validity criteria fulfilled:
yes
Conclusions:
The study was performed as a limit test at the nominal concentration of 100 mg/L under static conditions. The 24-hr and 48-hr EC50 were determined to be > 0.19 mg/L. These biological results were based on the geometric mean of the measured concentration.
Executive summary:

The acute toxicity of 1,3,5-triisopropylbenzen was tested under static conditions in a 48-h toxicity study on the water flea (Daphnia magna) following OECD TG 202. Analytical verification of the 100 mg/L test substance concentration reported a geometric mean measured concentration of 0.19 mg/L. Based on nominal concentrations, the 48-h EC50 was determined to be > 100 mg/L with a 48-h NOEC of 100 mg/L.

Description of key information

The acute toxicity of 1,3,5-triisopropylbenzen was tested under static conditions in a 48-h toxicity study on the water flea (Daphnia magna) following OECD TG 202. Analytical verification of the 100 mg/L test substance concentration reported a geometric mean measured concentration of 0.19 mg/L. Based on nominal concentrations, the 48-h EC50 was determined to be > 100 mg/L with a 48-h NOEC of 100 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information