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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
9 October 1985 - 18 Octobre 1985
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report date:
1985

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Principles and methods for their detections, modification mutagenic activity "Chemical Mutagens" T. Sugimura and M. Nagao, Vo1.6 pp.41-61 (1980) Plenum Press, (New York)
Deviations:
not specified
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
1-(5,6,7,8-tetrahydro-2-naphthyl)ethan-1-one
EC Number:
212-266-7
EC Name:
1-(5,6,7,8-tetrahydro-2-naphthyl)ethan-1-one
Cas Number:
774-55-0
Molecular formula:
C12H14O
IUPAC Name:
1-(5,6,7,8-tetrahydronaphthalen-2-yl)ethan-1-one
Test material form:
liquid
Details on test material:
Name: Acetyltetralin
Chemical name: 6-Acetyl-1,2,3,4-tetrahydronaphthalene
Appearance: colorless clear liquid

Method

Target gene:
Histidin
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
0, 10, 25, 50, 70, 100, 250 and 500 micrograms
Controls
Positive controls:
yes
Positive control substance:
2-nitrofluorene
sodium azide
N-ethyl-N-nitro-N-nitrosoguanidine
other: 2-Aminoanthracene; 9-Aminoacridine hydrochloride
Details on test system and experimental conditions:
After mixing the test m aterial, phosphate buffer or S-9 mix, culture of tester strain, the mixture was incubated under shaking at 37°C in a test tube for 20 minutes, top agar was added to the mixture and then the mixture was poured on minimal agar plate. The plates were incubated at 37°C for 2 days and scored for revertants.

Results and discussion

Test resultsopen allclose all
Key result
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Positive controls validity:
not specified
Key result
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Positive controls validity:
not specified
Key result
Species / strain:
E. coli WP2
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Positive controls validity:
not specified
Key result
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Positive controls validity:
not specified
Key result
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Positive controls validity:
not specified
Additional information on results:
See attachment for result tables.

Applicant's summary and conclusion

Conclusions:
The mutagenic activity of the test item was tested by the in vitro direct and metabolic acitivation assays using Salmonella typhimurium TA100, TA1535, TA1537, TA98 and
Escherichia coli WP-2. The test results indicated that the compound is not mutagenic under the test system used.