1-(5,6,7,8-tetrahydro-2-naphthyl)ethan-1-one

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

9 October 1985 - 18 Octobre 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Principles and methods for their detections, modification mutagenic activity "Chemical Mutagens" T. Sugimura and M. Nagao, Vo1.6 pp.41-61 (1980) Plenum Press, (New York)

Deviations:

not specified

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1-(5,6,7,8-tetrahydro-2-naphthyl)ethan-1-one

EC Number:

212-266-7

EC Name:

1-(5,6,7,8-tetrahydro-2-naphthyl)ethan-1-one

Cas Number:

774-55-0

Molecular formula:

C12H14O

IUPAC Name:

1-(5,6,7,8-tetrahydronaphthalen-2-yl)ethan-1-one

Test material form:

liquid

Details on test material:

Name: Acetyltetralin
Chemical name: 6-Acetyl-1,2,3,4-tetrahydronaphthalene
Appearance: colorless clear liquid

Method

Target gene:

Histidin

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

0, 10, 25, 50, 70, 100, 250 and 500 micrograms

Controls

Positive controls:

yes

Positive control substance:

2-nitrofluorene

sodium azide

N-ethyl-N-nitro-N-nitrosoguanidine

other: 2-Aminoanthracene; 9-Aminoacridine hydrochloride

Details on test system and experimental conditions:

After mixing the test m aterial, phosphate buffer or S-9 mix, culture of tester strain, the mixture was incubated under shaking at 37°C in a test tube for 20 minutes, top agar was added to the mixture and then the mixture was poured on minimal agar plate. The plates were incubated at 37°C for 2 days and scored for revertants.

Results and discussion

Test results

Test results 1

Key result

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Positive controls validity:

not specified

Test results 2

Key result

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Positive controls validity:

not specified

Test results 3

Key result

Species / strain:

E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Positive controls validity:

not specified

Test results 4

Key result

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Positive controls validity:

not specified

Test results 5

Key result

Species / strain:

S. typhimurium TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Positive controls validity:

not specified

Additional information on results:

See attachment for result tables.

Applicant's summary and conclusion

Conclusions:

The mutagenic activity of the test item was tested by the in vitro direct and metabolic acitivation assays using Salmonella typhimurium TA100, TA1535, TA1537, TA98 and
Escherichia coli WP-2. The test results indicated that the compound is not mutagenic under the test system used.