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EC number: 202-571-3 | CAS number: 97-30-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-06-22 to 2017-09-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 30 May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- LC-MS
- Details on sampling:
- - Concentrations: All five test concentrations
- Sample storage conditions before analysis: If analysis could not be applied immediately after sampling, samples were stored under circumstances (-20°C) to maintain the test item stable to the best of knowledge until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: The control consisted of dilution water only. Four replicates with five specimens per replicate were conducted.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no
- Method: The highest test concentration was prepared by adding 100.5 mg of the test item to 1 L dilution water. The solution was stirred using a magnetic stirring bar for about 1 hour at room temperature. The other test concentrations were prepared by serially dilution of the next higher test concentration starting with the highest test concentration of 100.5 mg/L using a spacing factor of 2. The test solutions were freshly prepared prior to test start. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna
- Source: German Federal Environment Agency, Institut für Wasser-, Boden- und Lufthygiene.
- Age of parental stock (mean and range, SD): at least 3 weeks
- Feeding during test: no
ACCLIMATION
- Acclimation period: adult Daphnids were acclimatised for 1 week
- Acclimation conditions (same as test or not): yes
- Type and amount of food: algal suspension (Desmodesmus subspicatus) and ArtemioFluid (JBL)
- Feeding frequency: Algae growing in the log-phase were centrifuged and the pellet was re-suspended in a few mL of medium. 30 mL of this suspension was given to 1 L Daphnia medium. The water was changed once per week.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Two Reading intervals: 24h and 48h
- Hardness:
- total hardness: 1.2 mmol/L
- Test temperature:
- 21.5°C ± 1°C
- pH:
- 8.22 – 8.55
- Dissolved oxygen:
- 8.1 mg/L - 9.0 mg/L
- Conductivity:
- 254 µS/cm
- Nominal and measured concentrations:
- Nominal: Control, 6.25, 12.5, 25, 50, 100 mg/L
measured: < LOQ, 6.37, 12.9, 23.3, 48.6, 96.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Type (delete if not applicable): The beakers were covered with glass panes to prevent from evaporation as much as possible, but also permit gaseous exchange between the medium and the atmosphere and access of light.
- Material, size, headspace, fill volume: Glass, 50 mL nominal volume, fill volume: 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified drinking water
- Total organic carbon: NPOC: 0.56 mg/L
- Ca/mg ratio: 2/3
- Conductivity: 254 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: not specified
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:16h light/8h darkness
- Light intensity: With 853 – 870 lx (corresponding to 11.4 – 11.6 µE/(m² * s)) the light intensity was below the maximum threshold value of about 1000 - 1500 lx (15 - 20 µE/(m² * s))
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation was determined in all groups after 24 and 48 hours.
RANGE-FINDING STUDY
- Test concentrations: 100.0, 10.0 and 1.0 mg/L nominal
- Results used to determine the conditions for the definitive study: In the first nominal test concentration of 1.0 mg/L one daphnid and in the highest test concentration of 100 mg/L four daphnids were found to be dead after 48 hours. However, this effect was not statistically significant. Test concentrations with a spacing factor of 2.0 were used in the definitive test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0%
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels:
Immobilisation after 24 h:
Control: 0 %
0.40 mg RS/L: 0 % related to control: 0 %
0.60 mg RS/L: 5 % related to control: 5 %
0.90 mg RS/L: 10 % related to control: 10 %
1.35 mg RS/L: 85 % related to control: 85 %
2.00 mg RS/L: 100 % related to control: 100 %
24 h EC50 value:
Immobilisation: 1.088 mg/L (95% CL :0.63 – 1.84) - Reported statistics and error estimates:
- No daphnid died up to and including the highest concentration of 100 mg a.s./L during the study. No mortality was observed in the control or in any the treatments. Therefore, no statistics were performed
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria as outlined under "any other information on material and methods" were fulfiled.
- Conclusions:
- Based on the results LOEC and NOEC are considered to be > 100 mg/L (EC0 = 100 mg/L) and alpha methyl glucoside is not regarded acutely toxic to Daphnia magna under the present experimental conditions.
- Executive summary:
The 48–hr-acute toxicity of alpha methyl glucoside to Daphnia magna was studied under static conditions. Daphnids were exposed to control, solvent control, and test chemical at arithmetic mean measured concentrations of 6.37, 12.9, 23.3, 48.6 and 96.1 mg a.s./L, corresponding to 101.9, 103.4, 93.3, 97.1 and 96.1 % of the nominal concentrations for 48 hr. Mortality/immobilization and sublethal effects were observed daily. The 48 – hr NOEC based on mortality and immobilization was > 100 mg a.i./L (EC0 = 100 mg/L). No sublethal effects were observed.
Test Organism Age : 0-24 h
Test Type: Static
NOEL> 100 mg a.i./L (EC0 = 100 mg/L)
Endpoint(s) Effected: None
Reference
Table 1: Effect of the test item on immobilisation of Daphnia magna
Nominal test item concentration [mg/L] |
measured test item concentration [mg/L] |
Immobilisation% |
|||||||||
24 h |
48 h |
||||||||||
|
|
Replicates |
Replicates |
||||||||
|
1 |
2 |
3 |
4 |
Mean |
1 |
2 |
3 |
4 |
Mean |
|
Control |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
|
6.25 |
6.37 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
12.5 |
12.9 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
25 |
23.3 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
50 |
48.6 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
96.1 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
Description of key information
- OECD guideline study (202), GLP, RL1, LOEC and NOEC > 100 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
In the present study conducted according to OECD guideline 202, each 20 daphnids (Daphnia magna) were incubated with the following nominal concentrations of the test item for 48 h: 0, 6.25, 12.5, 25, 50, 100 mg/L. The analytical verification of the concentrations revealed values which were between 80 and 120% of nominal test concentrations at test start and test end, thus, the evaluation of the concentration-effect-relationships and the calculations of effect concentrations were based on the nominal concentrations of the test item. Based on the results both LOEC and NOEC are considered to be > 100 mg/L and hence the test substance is not regarded acutely toxic to Daphnia magna under the present experimental conditions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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