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EC number: 942-655-3 | CAS number: 1802727-84-9
The toxicity of the test substance to the unicellular freshwater green alga Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata) was determined according to the principles of OECD 201 (2011). The aim of the study was the determination of NOEC, LOEC, EC10, EC20- and EC50-values of growth rate and yield over a period of 72 hours. The study was conducted under static conditions with an initial cell density of 10 000 cells/mL. The cell numbers were determined at 24, 48 and 72 hours after starting the test by manual cell counting using a microscope with counting chamber. The morphological changes of algal cells compared to the control were examined at 24, 48 and 72 hours after starting the test using a microscope. Based on the results of the non-GLP Preliminary Range-Finding Test the following five test concentrations in a geometric series (with a spacing factor of 3.0) were tested: 0.25, 0.74, 2.2, 6.7 and 20.0 mg/L (nominal concentrations). Untreated control ran parallel in the test and a positive control with potassium dichromate was performed. An appropriate amount of test item was dissolved in the dilution water (OECD medium) in order to obtain the concentration of 20 mg/L. The further test solutions were prepared by appropriate dilution of this stock solution. Samples were taken from the test concentrations and the control at the start and at the end of the experiment and analyzed by HPLC method with MS detection. Three parallel samples were analyzed from each test concentration and control at the start and at the end of the test. The measured concentration of the test item in the test solutions were 0.24, 0.72, 2.18, 6.29 and 20.8 mg/L (corresponding to 94 and 104 % of the nominal value) at the start of the test and 0.22, 0.70, 2.16, 6.46 and 19.5 mg/L (corresponding to 90 and 98 % of the nominal value) at the end of the test. The concentration of the tested substance was maintained within ± 20 % of the nominal concentrations throughout the test. Therefore, the analysis of the results was based on the nominal concentration values. The 24h-ErC50 and the EyC50 of potassium dichromate was determined to be 0.82 and 0.51 mg/L respectively. The validity criteria were fulfilled. After 72 hours the ErC10 was determined to be 2.5 mg/L and the ErC50 was determined to be 9.31 mg/L.
The toxicity of the test substance to the unicellular freshwater green alga Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata) was determined according to the principles of OECD 201 (2011) under GLP conditions. After 72 hours the ErC10 was determined to be 2.5 mg/L and the ErC50 was determined to be 9.31 mg/L.
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