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EC number: 942-655-3 | CAS number: 1802727-84-9
The purpose of this study was to determine the ready biodegradability of the test item. The test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. The test item was investigated at the concentration of 3.0 mg/L. The concentration was chosen based on the preliminary test results and based on the theoretical oxygen demand of 2.44 mg O2/mg test item (ThODNH4, calculated according to equation given in the guidelines, assuming that no nitrification occurs). In parallel (under the same conditions as the test item), positive reference item, sodium benzoate at the concentration of 3.0 mg/L (as procedure control), inoculum control and toxicity control were investigated. All validity criteria of the study were met. Under the test conditions ready biodegradation of this test item was not noticed. The percentage biodegradation of the test item reached a mean of 4.7 % after 28 days based on its ThODNH4. The parallel running analytical determination of a possible nitrite and nitrate development demonstrated that no nitrification occurred, therefore the biodegradability of the test item was calculated based on its ThODNH4; any correction, based on the nitrite and/or nitrate content was not performed. The reference item sodium benzoate was sufficiently degraded to a mean of 72.4 % after 14 days, and to a mean of 83.8 % after 28 days of incubation, based on ThODNH4. In the toxicity control containing both, the test item and the reference item, a mean of 27.2 % biodegradation was noted within 14 days and 31.5 % biodegradation after 28 days of incubation. The test item is considered to be not ready biodegradable, since the pass level for ready biodegradability is removal of 60% ThODNH4 in a 10-day window. The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum. According to the test guidelines the test item can be assumed as not inhibitory at the applied concentration level on the activated sludge microorganisms because the degradation in the toxicity control group was higher than 25 % within 14 days.
The test item was considered to be not ready biodegradable.
The test item was considered to be not ready biodegradable. According to the test guidelines the pass level for ready biodegradability is 60 % of ThODNH4. Under the test conditions the percentage biodegradation of the test item reached a mean of 4.7 % after 28 days based on its calculated ThODNH4. Any correction of the BOD values with the observed nitrite values was considered as not necessary. According to the test guidelines the test item can be assumed as not inhibitory on the activated sludge microorganisms because the degradation in the toxicity control group was higher than 25 % within 14 days. The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum.
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