Hydroxylamine-O-sulphonic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from publication.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

MUTAGENICITY AND DNA- MODIFYING ACTIVITY: A COMPARISON OF TWO MICROBIAL ASSAYS

Author:

HERBERT S. ROSENKRANZ, BEZALEL GUTTER and WILLIAM T. SPECK

Year:

1976

Bibliographic source:

Mutation Research, 41 (1976) 61—70

Reference 2

Reference Type:

publication

Title:

Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity test

Author:

Ames BN, McCann J, Yamasaki E

Year:

1975

Bibliographic source:

Mutation Research, 31, 347-364

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

To evaluate the mutagenic potential of Hydroxylamine-O-sulfonic acid in Salmonella typhimurium strains TAI535 and TAI538 by using Standard plate incorporation assay

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Hydroxylamine-O-sulphonic acid

EC Number:

220-971-6

EC Name:

Hydroxylamine-O-sulphonic acid

Cas Number:

2950-43-8

Molecular formula:

H3NO4S

IUPAC Name:

Hydroxylamine-O-sulphonic acid

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material (IUPAC name): Hydroxylamine-O-sulphonic acid
- Molecular formula: H3NO4S
- Molecular weight: 113.093 g/mol
- Smiles notation: O=S(=O)(ON)O
- InChl: 1S/H3NO4S/c1-5-6(2,3)4/h1H2,(H,2,3,4)
- Substance type: Organic
- Physical state: Solid

Specific details on test material used for the study:

- Name of test material (IUPAC name): Hydroxylamine-O-sulphonic acid
- Molecular formula: H3NO4S
- Molecular weight: 113.093 g/mol
- Smiles notation: O=S(=O)(ON)O
- InChl: 1S/H3NO4S/c1-5-6(2,3)4/h1H2,(H,2,3,4)
- Substance type: Organic
- Physical state: Solid

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TAI535 and TAI538

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9 mix - liver homogenates(S-9) (9000 g supernatant) from rats induced with a polychlorinated biphenyI (PCB) mixture (Aroclor 1254)

Test concentrations with justification for top dose:

0.1µl

Vehicle / solvent:

Not specified

Controls

Untreated negative controls:

yes

Remarks:

Water

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

methylmethanesulfonate

Details on test system and experimental conditions:

Details on test system and conditions
METHOD OF APPLICATION: Standard plate incorporation assay
DURATION
- Exposure duration: 48 hour

Rationale for test conditions:

Not specified

Evaluation criteria:

Numbers of Histidine revertants per plates were observed.

Statistics:

Yes, Mean was observed

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium, other: TAI535 and TAI538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: No mutagenic effect were observed

Any other information on results incl. tables

	Agent	Amount	Revertants per plate
			TA1535	TA1538
Test chemical	Hydroxylamine-o-sulfonic acid (HOMS)	0.1µl	44	16
Positive control	Methyl methanesulfonate	10 µl	439	30
Negative Control	water	10 µl	13	10

Applicant's summary and conclusion

Conclusions:

Hydroxylamine-O-sulfonic acid was evaluated for its mutagenic potential in Salmonella typhimurium strains TAI535 and TAI538 by using Standard plate incorporation assay. The test result was considered to be negative in the presence and absence of S9.

Executive summary:

Genetic toxicity in vitro study of Hydroxylamine-O-sulfonic acid was assessed for its possible mutagenic potential. For this purpose is AMES assay was performed in Salmonella typhimurium strains TAI535 and TAI538 by usingStandard plate incorporation assay. The test chemical was used at the concentration of 0.1µl/plate in the presence and absence of S9 mix. No mutagenic effects were observed. Therefore Hydroxylamine-O-sulfonic acid was considered to be non mutagenic in Salmonella typhimurium strains TAI535 and TAI538 with and without metabolic activation. Hence the substance cannot be classified as gene mutant in vitro.