Hydroxylamine-O-sulphonic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental results of read across chemicals

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE report is prepared based on short term toxicity to aquatic invertebrates study.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): Hydroxylamine-O-sulphonic acid
- Molecular formula: H3NO4S
- Molecular weight: 113.093 g/mol
- Smiles notation: O=S(=O)(ON)O
- InChl: 1S/H3NO4S/c1-5-6(2,3)4/h1H2,(H,2,3,4)
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Aquatic shrimps and daphnia magna

Details on test organisms:

2 and 3. Details on test organisms
TEST ORGANISM
- Common name: Water flea

Test type:

static

Water media type:

other: Study 1 Aerated water and 2 freshwater

Total exposure duration:

48 h

Post exposure observation period:

24 hrs

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 80 - <= 90 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Other details not known

Duration:

1 d

Dose descriptor:

EC50

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Remarks on result:

other: Changes in behavior was on equillibrium

Validity criteria fulfilled:

not specified

Conclusions:

1. After the exposure of test chemical with the shrimps for 48 hrs, lethal effect (LC50) were measured and determine to be 80-90 mg/l.
2. Based on the changes observed in the daphnia magna by the chemical exposure for 24 hrs, the EC50 was determine to be 32 mg/l.
3. Based on the immobility of daphnia magna by the chemical exposure for 48 hrs, the IC50 was determine to be 12 mg/l.

Executive summary:

Short term toxicity of Hydroxylamine-O-sulphonic acid (2950 -43 -8) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates. Study was conducted to evaluate the toxicity of test chemical on the growth of shrimps. Study conducted under the static system in aerated water for 48 hrs. After the exposure of test chemical with the shrimps for 48 hrs, lethal effect (LC50) were measured and determine to be 80-90 mg/l. Based on the LC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Similar study was conducted for another read across chemical. Aim of this study was to determine the action and effect of chemical when exposed with the daphnia magna. Study was conducted for 24 hrs under the static system. After the incubation period effect on 50 % population of test organism occurs were observed. Based on the changes observed in the daphnia magna by the chemical exposure for 24 hrs, the EC50 was determine to be 32 mg/l. Based on the EC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

In third weight of evidence study for structurally and functionally similar read across chemicals toxicity was measured. Aim of this study was to determine the action and effect of chemical when exposed with the daphnia magna. Test conducted in static system for 2 days. Based on the immobility of daphnia magna by the chemical exposure for 48 hrs, the IC50 was determine to be 12 mg/l. Based on the IC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Thus based on the overall studies for the structurally and functionally similar read across chemical for Hydroxylamine-O-sulphonic acid (2950 -43 -8), it can be concluded that the test chemical was toxic as value ranges from 12 -90 mg/l and classified as aquatic chronic 3 as per the CLP classification criteria.  

 

Description of key information

1. After the exposure of test chemical with the shrimps for 48 hrs, lethal effect (LC50) were measured and determine to be 80-90 mg/l.

2. Based on the changes observed in the daphnia magna by the chemical exposure for 24 hrs, the EC50 was determine to be 32 mg/l.

3. Based on the immobility of daphnia magna by the chemical exposure for 48 hrs, the IC50 was determine to be 12 mg/l.

Based on the above data chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

90 mg/L

Additional information

Short term toxicity of Hydroxylamine-O-sulphonic acid (2950 -43 -8) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it closely related read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates. Study was conducted to evaluate the toxicity of test chemical on the growth of shrimps. Study conducted under the static system in aerated water for 48 hrs. After the exposure of test chemical with the shrimps for 48 hrs, lethal effect (LC50) were measured and determine to be 80-90 mg/l. Based on the LC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Similar study was conducted for another read across chemical. Aim of this study was to determine the action and effect of chemical when exposed with the daphnia magna. Study was conducted for 24 hrs under the static system. After the incubation period effect on 50 % population of test organism occurs were observed. Based on the changes observed in the daphnia magna by the chemical exposure for 24 hrs, the EC50 was determine to be 32 mg/l. Based on the EC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

In third weight of evidence study for structurally and functionally similar read across chemicals toxicity was measured. Aim of this study was to determine the action and effect of chemical when exposed with the daphnia magna. Test conducted in static system for 2 days. Based on the immobility of daphnia magna by the chemical exposure for 48 hrs, the IC50 was determine to be 12 mg/l. Based on the IC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Thus based on the overall studies for the structurally and functionally similar read across chemical for Hydroxylamine-O-sulphonic acid (2950 -43 -8), it can be concluded that the test chemical was toxic as value ranges from 12 -90 mg/l and classified as aquatic chronic 3 as per the CLP classification criteria.