Hydroxylamine-O-sulphonic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06.11.2018 - 10.11.2018

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the OECD guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound Hydroxylamine-O-sulphonic acid on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): Hydroxylamine-O-sulphonic acid
- Molecular formula: H3NO4S
- Molecular weight: 113.093 g/mol
- Smiles notation: O=S(=O)(ON)O
- InChl: 1S/H3NO4S/c1-5-6(2,3)4/h1H2,(H,2,3,4)
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

Method: The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish(Danio rerio)
- Source: Anytime Pet, Jariphataka, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.4 cm
- Weight at study initiation (mean and range, SD): 0.045g (average)


ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): Same as test
- Feeding frequency during acclimation: Daily once
- Health during acclimation (any mortality observed): No mortality were observed

Other conditions during acclimation:
- Photoperiod 16 hours light and 8 hours dark
- Temperature range 23.9 °C
- pH range 7.5
- DO range 7.6 mg/l
- Aeration Continous

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

24, 48, 72, 96 hrs

Hardness:

155.5 (mg of CaCO3)

Test temperature:

23 °C

pH:

7.2

Dissolved oxygen:

7.6 mg/l

Nominal and measured concentrations:

6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: Plastic aquaria having the water capacity of 5 liters filled with 2 liters of water with 3 liter of headspace.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 2


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light and 8 hours dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations of (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 6.25 - <= 12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No effect were observed at 6.25 mg/l.

Key result

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Toxic

Sublethal observations / clinical signs:

Fig:Mortality and symptoms

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3hr			6hr		24hr			48hr		72hr			96hr
	4		V1	V2		V1	V2	V1	V2		V1	V2	V1	V2		V1	V2
Control	4	4	-	-		-	-	-	-		-	-	-	-		-	-
6.25	4	4	-		-	-	-	-		-	-	-	-		-	-		-
12.5	4	4	-		-	-	-	4		4	4	4	4		4	4		4
25	4	4	-		-	-	-	4		4	4	4	4		4	4		4
50	4	4	-		-	4	4	4		4	4	4	4		4	4		4
100	4	4	4		4	4	4	4		4	4	4	4		4	4		4

Fig: Visible Symptoms:

 

Nominal Concentration (mg/l)	Number of fish		symptom (cumulated) after
			3hr		6hr		24hr			48hr		72hr			96hr
	4	4	V1	V2	V1	V2	V1	V2		V1	V2	V1	V2		V1	V2
Control	4	4	NS4	NS4	NS4	NS4	NS4	NS4		NS4	NS4	NS4	NS4		NS4	NS4
6.25	4	4	NS4	NS4	NS4	NS4	NS4		NS4	NS4	NS4	NS4		NS4	NS4	NS4
12.5	4	4	NS4	NS4	NS4	NS4
25	4	4	NS4	NS4	NS4	NS4
50	4	4	NS4	NS4	-	-	-		-	-	-	-		-	-	-
100	4	4	NS4	NS4	-	-	-		-	-	-	-		-	-	-

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

 

 

 

 

 

8.2. pH and oxygen content:

 

		Number of	pH readings after
Nominal	fish		24h		48h		72h		96h
Concentration			V1	V2	V1	V2	V1	V2	V1	V2
(mg/l)
Control	4	4	7.7	7.7	7.8	7.8	7.9	7.9	8.0	8.0
6.25	4	4	7.9	7.9	8.0	8.0	8.1	8.1	8.2	8.2
12.5	4	4	-	-	-	-	-	-	-	-
25	4	4	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-

 

Nominal	Number of		Oxygen content (mg/L) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.0	7.0	6.9	6.9	6.8	6.8	6.7	6.7
6.25	4	4	6.8	6.8	6.7	6.7	6.5	6.5	6.5	6.5
12.5	4	4	-	-	-	-	-	-	-	-
25	4	4	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-
8.3. Test temperature
Nominal	Number of		Temperature (°C) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	23.1	23.1	23.2	23.2	23.0	23.0	22.9	22.9
6.25	4	4	23.1	23.1	23.2	23.2	23.0	23.0	22.9	22.9
12.5	4	4	-	-	-	-	-	-	-	-
25	4	4	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-

 

 

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Hydroxylamine-O-sulphonic acid on Zebra fish (Danio rerio) was determined to be 12.5 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Executive summary:

This study was designed to assess the toxic effects of the test compound Hydroxylamine-O-sulphonic acid on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.

Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.  

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Hydroxylamine-O-sulphonic acid on Zebra fish (Danio rerio) was determined to be 12.5 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Hydroxylamine-O-sulphonic acid on Zebra fish (Danio rerio) was determined to be 12.5 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

12.5 mg/L

Additional information

Toxicity of chemical was determine and studied on the growth of fish (experimental study report 2018). This study was designed to assess the toxic effects of the test compound Hydroxylamine-O-sulphonic acid on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Hydroxylamine-O-sulphonic acid on Zebra fish (Danio rerio) was determined to be 12.5 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.