Butyl propionate

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The study was conducted as per the method outlined in the publication - Alsop, G.M.; Waggy, G.T.; Conway, R.A., Bacterial Growth Inhibition Test, Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980 and the report contains sufficient information to permit a meaningful evaluation of study results

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Alsop, G.M.; Waggy, G.T.; Conway, R.A., Bacterial Growth Inhibition Test, Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980

Deviations:

no

Principles of method if other than guideline:

not applicable

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Butyl propionate

EC Number:

209-669-5

EC Name:

Butyl propionate

Cas Number:

590-01-2

Molecular formula:

C7H14O2

IUPAC Name:

butyl propanoate

Details on test material:

- Name of test material (as cited in study report): n-butyl propionate

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

not applicable

Test solutions

Vehicle:

not specified

Details on test solutions:

no data

Test organisms

Test organisms (species):

other: no data

Details on inoculum:

no data

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Post exposure observation period:

not applicable

Test conditions

Hardness:

no data

Test temperature:

22 ± 2 °C

pH:

no data

Dissolved oxygen:

no data

Salinity:

not applicable

Nominal and measured concentrations:

no data

Details on test conditions:

The study was conducted as per the method outlined in the publication - Alsop, G.M.; Waggy, G.T.; Conway, R.A., Bacterial Growth Inhibition Test, Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980. The test material n-butyl propionate was evaluated at selected conentrations in a mixture containing buffer, nutrients, growth substrate and microorganisms. The mixture of 1 ml of a suspension of seed microorganisms, 20 ml of dilution water, 4 ml of stock buffer solution, 10 ml of yeast extract/sodium acetate solution and 4 ml of aqueous solution of n-butyl propionate were incubated in an 8-ounce, narrow neck, round bottle for 16 hours on a platform shaker at ambient temperature (22 ± 2 °C). Seeded control bottles were used to measure growth or turbidity generated during the 16 hours without the test material. The bottles were stoppered with cotton plugs during shaking to avoid contamination.
The degree of inhibition was assessed from measuring (optical density at 530 nm) the turbidity levels of the test material at various concentrations. The measured optical density values were calculated as a percentage of the seeded growth control system. The percentage of control values were then plotted against the log of test sample concentration. The test concentration corresponding to 50 percent of the control was termed as 50 percent inhibition concentration (IC50).

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

16 h

Dose descriptor:

IC50

Effect conc.:

508 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: turbidity measurements

Details on results:

no data

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

no data

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of the study, the IC50 of n-butyl propionate was 508 mg/l.

Executive summary:

The study was conducted as per the method outlined in the publication - Alsop, G.M.; Waggy, G.T.; Conway, R.A., Bacterial Growth Inhibition Test, Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980. The test material n-butyl propionate was evaluated at selected conentrations in a mixture containing buffer, nutrients, growth substrate and microorganisms. The mixture of 1 ml of a suspension of seed microorganisms, 20 ml of dilution water, 4 ml of stock buffer solution, 10 ml of yeast extract/sodium acetate solution and 4 ml of aqueous solution of n-butyl propionate were incubated in an 8-ounce, narrow neck, round bottle for 16 hours on a platform shaker at ambient temperature (22 ± 2 °C). Seeded control bottles were used to measure growth or turbidity generated during the 16 hours without the test material. The bottles were stoppered with cotton plugs during shaking to avoid contamination.

The degree of inhibition was assessed from measuring (optical density at 530 nm) the turbidity levels of the test material at various concentrations. The measured optical density values were calculated as a percentage of the seeded growth control system. The percentage of control values were then plotted against the log of test sample concentration. The test concentration corresponding to 50 percent of the control was termed as 50 percent inhibition concentration (IC50). Under the conditions of the study, the IC50 of n-butyl propionate was 508 mg/l.