Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-)

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on daphnia magna was predicted for Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-) (6408-31-7). Based on the intoxication of test organism the EC50 value was estimated to be 157.929 mg/l when Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1 -sulphonato(4-)]hydroxychromate(2-) exposed to daphnia magna for 48 hrs. Based on this value it can be concluded that the substance Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)] hydroxychromate(2-) is considered to be not toxic to aquatic environment as per the criteria mentioned in CLP regulation.   

 

Toxicity to algae and cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, toxicity was predicted for Disodium [5-chloro-3- [[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato (4-)] hydroxychromate(2-) (6408-31-7). Based on the growth rate inhibition of Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) by the chemical Disodium [5-chloro-3-[[4,5-dihydro-3-methyl -5-oxo-1-(3-sulphophenyl) -1H-pyrazol-4-yl]azo]-2-hydroxybenzene- 1-sulphonato(4-)]hydroxychromate(2-) the EC50 was 129.0149 mg/l. Based on this value it can be concluded that the substance Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene -1-sulphonato(4-)] hydroxychromate(2-) is considered to be not toxic to aquatic environment and not classified as per the criteria mentioned in CLP regulation.   

 

Additional information

Short term toxicity to aquatic invertebrates:

Various predicted data for the target compound Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-) (6408-31-7) and supporting weight of evidence studies for its closest read across substance with log Kow as the primary descriptor were reviewed for the toxicity on the invertebrates end point which are summarized as below: 

In a prediction done by SSS (2017), Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on daphnia magna was predicted for Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-) (6408-31-7). Based on the intoxication of test organism the EC50 value was estimated to be 157.929 mg/l when Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1 -sulphonato(4-)]hydroxychromate(2-) exposed to daphnia magna for 48 hrs. Based on this value it can be concluded that the substance Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)] hydroxychromate(2-) is considered to be not toxic to aquatic environment as per the criteria mentioned in CLP regulation.   

 

In second prediction using the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted. On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 892.348 mg/l for Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1- (3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-) in 48 hrs. Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.

 

Similarly in a supporting weight of evidence study from Ecotoxicology and Environmental Safety, 1999 for read across chemical (1934-21-0) study were to determine the toxicity of a chemical Trisodium 5-hydroxy-1-(4-sulphophenyl)-4-(4-sulphophenylazo)pyrazole-3-carboxylate (Tartrazine; Food yellow 4) on the growth of water flea Ceriodaphnia cf. dubia. Stock solutions were made by dissolving the appropriate amount in 1 or 2 L of the water described previously and then gently stirred for 12 h in the dark at 23 ± 1°C using Teflon magnetic stirrers. Light was excluded during the stirring to minimize photodegradation of the chemicals. Stock solutions were diluted to the appropriate concentrations immediately prior to the commencement of a test. Test was conducted on the < 24hrs old water flea. 250-ml glass beakers which held 200 ml of the test solution or control solution having headspace of 50 ml were used. The absence of visible movement by the cladocera within 15 s of gentle agitation of the test solution was observed at 5706.55 mg/l. Based on the immobilization of Ceriodaphnia cf. dubia due to the exposure of chemical Trisodium 5-hydroxy-1-(4-sulphophenyl)-4-(4-sulphophenylazo)pyrazole-3-carboxylate, the EC50 was 5706.55 mg/l. Thus based on the EC50, the chemical was consider as nontoxic and can be consider to be not classified.

 

Similarly fourth study was conducted on the read across chemical selected on the basis of structure similarity (1934-21-0) from The Journal of Toxicological Sciences, 1977. Short term toxicity study to Artemia Salina was carried out for 24-48 hrs. The test chemical conc. used for the study was 5343.68 mg/l and 534.368 mg/l, respectively. A. salina eggs (encysted dried gastrulae) were commercially obtained, and were stored at -20°C, Eggs used in experiments were washed and stored at room temperature in a desiccator over anhydrous granular CaCl, . Larvae were obtained by incubating eggs in petri dishes containing muslin-filtered sea water at 30°C for 24 hours. The larvae were separated from shells, dead larvae and unhatched eggs by their phototactic movements toward a light source. Food dyes of various concentrations were placed in a petri dish, and sea water containing 20 to 30 larvae was added. After this was incubated at 30°C for 24 hours and 48 hours, larvae surviving were measured by direct count. The same method was tested from 5 to 6 times for each concentration, and the death rate was calculated. The study was performed under static conditions for 24 – 48 hrs at 30°C. Death was assumed to have occurred when there was no movement. The death rate was defined as the average of the percentage of deaths observed for 24 hours and 48 hours. Based on death rate or mortality of test organism, the LC83.8 value was found to be 5343.68 mg/l. The chemical was considered as not classified according to the CLP regulation.

On the basis of above results for target chemical Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-) (6408-31-7) (from OECD QSAR toolbox version 3.4, and EPIsuite, 2017) and for its read across substance from peer reviewed journal Ecotoxicology and Environmental Safety, 1999 and The Journal of Toxicological Sciences, 1977, it can be concluded that the test substance Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-) is not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Toxicity to algae and cyanobacteria:

Various predicted data for the target compound Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]- 2-hydroxybenzene-1-sulphonato(4-) ]hydroxychromate(2-) (6408-31-7) and supporting weight of evidence studies for its closest read across substance with log Kow as the primary descriptor were reviewed for the toxicity on the algae and cyanobacteria end point which are summarized as below: 

 

In a prediction done by SSS (2017), Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, toxicity was predicted for Disodium [5-chloro-3- [[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato (4-)] hydroxychromate(2-) (6408-31-7). Based on the growth rate inhibition of Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) by the chemical Disodium [5-chloro-3-[[4,5-dihydro-3-methyl -5-oxo-1-(3-sulphophenyl) -1H-pyrazol-4-yl]azo]-2-hydroxybenzene- 1-sulphonato(4-)]hydroxychromate(2-) the EC50 was 129.0149 mg/l. Based on this value it can be concluded that the substance Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene -1-sulphonato(4-)] hydroxychromate(2-) is considered to be not toxic to aquatic environment and not classified as per the criteria mentioned in CLP regulation.   

 

 

In second prediction using prediction done using theEPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance Disodium [5-chloro-3- [[4,5-dihydro-3-methyl-5-oxo- 1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-) (6408 -31 -7). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 510.661 mg/l for green algae for 96 hrs of duration. Based on this value, it can be concluded that the test chemical Disodium [5-chloro-3-[[4,5- dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]- 2-hydroxybenzene-1-sulphonato(4-)] hydroxychromate(2-) can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

 

Similarly in a supporting weight of evidence study from ABITEC lab report 2017, for the read across chemical trisodium 2-({6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)(methyl)amino]-1- hydroxy-3-sulfonato-2-naphthyl}diazenyl)naphthalene-1,5-disulfonate (70616-89-6) which was structurally and functionally similar to the target chemical was used. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance trisodium 2-({6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)(methyl) amino]-1-hydroxy- 3-sulfonato- 2-naphthyl}diazenyl)naphthalene-1,5-disulfonate according to OECD Guideline 201. The stock solution 100 mg/l was prepared by dissolving redbrown powder in OECD growth medium and tested at the limit concentration i.e 100 mg/l. Effects on the growth rate of the organism were studied after the exposure period of 72hrs with the positive reference control Potassium dichromate (K2Cr2O7). Test performed in the six replicates. The inhibitory concentration for the test substance, trisodium 2-({6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)(methyl) amino]-1-hydroxy-3-sulfonato-2-naphthyl}diazenyl) naphthalene-1,5- disulfonate, in Desmodesmus subspicatus was determined. At 100 mg/l inhibition was not observed in any replicates (0% inhibition was observed). This value indicates that the substance is likely to be non-hazardous to aquatic algae and can be consider as not classified as per the CLP criteria.

 

On the basis of above results for target chemical Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2- hydroxybenzene-1 -sulphonato(4-) hydroxychromate(2-) (6408-31-7) (from OECD QSAR toolbox version 3.4, and EPIsuite, 2017) and for its read across substance from ABITEC report 2017, it can be concluded that the test substance Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene-1-sulphonato(4-)]hydroxychromate(2-) is not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Based on the toxicity on algae and invertebrates it can be concluded that the substance Disodium [5-chloro-3-[[4,5-dihydro-3-methyl-5-oxo-1-(3-sulphophenyl)-1H-pyrazol-4-yl]azo]-2-hydroxybenzene -1-sulphonato(4-)] hydroxychromate(2-) is considered to be not toxic to aquatic environment and not classified as per the criteria mentioned in CLP regulation.