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Short-term toxicity to aquatic invertebrates

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Description of key information

Exposure of Daphnia magna to the test item gave an EL50 value of 49 mg/L loading rate WAF (confidence limits 42-58 mg/L loading rate WAF). The No Observed Effect Loading Rate was 18 mg/L loading rate WAF and the Lowest Observed Effect Loading Rate (LOELR) was 32 mg/L (OECD 202 and EU Method C.2).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
49 mg/L

Additional information

GUIDELINE

A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202,"Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.

 

METHODS

Due to the low aqueous solubility and complex nature of the test item, for the purposes of the test, the test medium was prepared as a Water Accommodated Fraction (WAF). Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 10,18, 32, 56 and 100 mg/L for 48 hours at a temperature of 21 °C to 22 °C under static test conditions. The number of immobilised daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.

 

RESULTS

Total Organic Carbon (TOC) analysis of the fresh test preparations at 0 hours showed measured concentrations to range from 2.8 to 30 mg C/L. Analysis of the aged test preparations at 48 hours showed measured concentrations to range from 2.6 to 30 mg C/L. The dissolved test item may have been one or several components of the test item. Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole, the results were based on nominal loading rates only.

 

CONCLUSION

Exposure of Daphnia magna to the test item gave an EL50 value of 49 mg/L loading rate WAF (confidence limits 42-58 mg/L loading rate WAF). The No Observed Effect Loading Rate was 18 mg/L loading rate WAF and the Lowest Observed Effect Loading Rate (LOELR) was 32 mg/L.