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EC number: 946-517-3
CAS number: -
growth rate, the EL50 (72h) value was determined to be 14
mg/L loading rate WAF based on growth rate and the NOEC (72h) was
reported as 3.2 mg/L loading rate WAF based on growth rate (OECD 201 and
EU Method C.3).
was performed to assess the effect of the test item on the growth of the
subcapitata. The method followed
was designed to be compatible with the OECD Guidelines for Testing of
Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth
Inhibition Test" referenced as Method C.3 of Commission Regulation (EC)
to the low aqueous solubility and complex nature of the test item for
the purposes of the test the test item was prepared as a Water
Accommodated Fraction (WAF). Following preliminary range-finding tests,
Pseudokirchneriella subcapitata was exposed to Water Accommodated
Fractions (WAFs) of the test item at nominal loading rates of 1.0, 3.2,
10, 32 and 100 mg/L (three replicate flasks per concentration) for 72
hours, under constant illumination and shaking at a temperature of 24 ±
1 °C. Samples of the algal populations were removed daily and cell
concentrations determined for each control and treatment group, using a
Coulter Multisizer Particle Counter.
Organic Carbon (TOC) analysis of the test preparations performed at 0
and 72 hours showed measured concentrations of 3.0 to 18 mg C/L at 0
hours and 1.6 to 29 mg C/L at 72 hours. Given that the toxicity cannot
be attributed to a single component or a mixture of components but to
the test item as a whole, the results were based on nominal loading
of Pseudokirchneriella subcapitata to the test item gave EL50 values of
14 mg/L loading rate WAF (confidence limits 11 to 18 mg/L loading rate
WAF) based on growth rate and 4.2 mg/L loading rate WAF (confidence
limits 3.6 to 4.9 mg/L loading rate WAF) based on yield. The No Observed
Effect Loading Rate was 3.2 mg/L loading rate WAF based on growth rate
and 1.0 mg/L loading rate WAF based on yield. The Lowest Observed Effect
Loading Rate was 10 mg/L based on growth rate and 3.2 mg/L based on
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