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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical 3-Methylbutyl propanoate on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Anytime pet, Jaripatka, Nagpur.
- Length at study initiation (length definition, mean, range and SD): average 1.88 cm
- Weight at study initiation (mean and range, SD): average 0.0792 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
200 mg of CaCO3
Test temperature:
24 °C
pH:
6.9
Dissolved oxygen:
6.9 mg/l
Nominal and measured concentrations:
2.375, 4.75, 9.5, 19 and 38 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4.75 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other:
Sublethal observations / clinical signs:

Table 1: Mortality

Nominal Concentration

(mg/l)

Number of fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

V1

V1

V1

V1

V1

V1

Control

8

-

-

-

-

-

-

2.375

8

-

-

-

-

-

-

4.75

8

-

-

1

1

1

2

9.5

8

-

-

4

4

4

4

19

8

-

-

4

4

4

4

38

8

4

4

4

4

4

4

 

 

Table 2: Symptoms

Nominal Concentration

(mg/l)

Number of fish

Symptom (cumulated) after

3h

6h

24h

48h

72h

96h

V1

V1

V1

V1

V1

V1

Control

8

NS4

NS4

NS4

NS4

NS4

NS4

2.375

8

NS4

NS4

NS4

NS4

NS4

NS4

4.75

8

NS4

M4

M3

M3

M3

M2

9.5

8

M4

M4

-

-

-

-

19

8

M4

M4

-

-

-

-

38

8

-

-

-

-

-

-

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

E: Exopthalamos

SC: Schooling Effect

 

Table 3: pH

Nominal Concentration

(mg/l)

Number of fish

pH readings after

 

24h

48h

72h

96h

V1

V1

V1

V1

Control

8

7.3

7.4

7.5

7.6

2.375

8

7.5

7.6

7.8

7.9

4.75

8

7.6

7.7

7.9

8.0

9.5

8

-

-

-

-

19

8

-

-

-

-

38

8

-

-

-

-

 

Table 4: oxygen content

Nominal Concentration

(mg/l)

Number of fish

Oxygen content (mg/L) after

 

24h

48h

72h

96h

V1

V1

V1

V1

Control

8

6.9

6.8

6.7

6.6

2.375

8

6.7

6.6

6.5

6.4

4.75

8

6.7

6.6

6.5

6.4

9.5

8

-

-

-

-

19

8

-

-

-

-

38

8

-

-

-

-

Table 5: Test temperature

Nominal Concentration

(mg/l)

Number of fish

Temperature (°C) after

 

24h

48h

72h

96h

V1

V1

V1

V1

Control

8

23.7

23.6

23.8

24.1

2.375

8

23.7

23.6

23.8

24.1

4.75

8

23.7

23.6

23.8

24.1

9.5

8

-

-

-

-

19

8

-

-

-

-

38

8

-

-

-

-

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.105-68-0 on Zebra Fish Danio rerio was determine to be 4.75 mg/l.
Executive summary:

Study was conducted to assess the effect of test chemical3-Methylbutyl propanoateon the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio rerio were exposed to these concentrations for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item (3-Methylbutyl propanoate) to various nominal test concentrations, LC50 was determine to be (4.75 ) mg/l . Based on the LC50, it can be consider that the chemical was hazardous and can be consider to be classified aquatic chronic 2 as per the CLP classification criteria.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.105-68-0 on Zebra Fish Danio rerio was determine to be 4.75 mg/l.  

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
4.75 mg/L

Additional information

Study was conducted to assess the effect of test chemical 3-Methylbutyl propanoateon the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio rerio were exposed to these concentrations for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item (3-Methylbutyl propanoate) to various nominal test concentrations, LC50 was determine to be (4.75 ) mg/l . Based on the LC50, it can be consider that the chemical was hazardous and can be consider to be classified aq aquatic chronic 2 as per the CLP classification criteria.