Registration Dossier
Registration Dossier
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EC number: 203-142-3 | CAS number: 103-76-4
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriately low concentrations.
Key value for chemical safety assessment
Additional information
The toxicity to microorganisms was assessed in a weight-of-evidence approach based on two experimental studies. One of these studies was not performed with the pure test substance, but with a mixture of piperazines.
A study on inhibitory effects of hydroxyethylpiperazine (HEP; CAS 103 -76 -4) on a single microbial species was conducted according to ISO 10712 (Pseudomonas, cell multiplication inhibition test; Akzo-Nobel, 1996; report no.: 142/267). Based on nominal test concentrations, the 16-h EC10 and 16-h EC50 were determined to be 14 mg/L and 100 mg/L, respectively. However, according to the REACh guidance document R.7b (v4.0, ECHA, 2017), the results of the cell multiplication inhibition test with P. putida (Bringmann and Kühn, 1980; ISO 10712, 1996) should be used for calculation of the PNEC(STP) only in cases where no other test results are available. Preference is given to tests with a mixed inoculum.
The second study was performed with hydroxyethylpiperazine as part of a mixture (approximately 38% HEP). Short-term effects of the hydroxyethylpiperazine mixture (piperazine: 14.57 %, HEP: 37.768 %, DiHEP: 19.90 %) on the respiration rate of domestic activated sludge were studied in an OECD 209 GLP guideline study. No inhibitory effects were observed. The 3-h EC10 was > 1000 mg/L (BASF, 2012; report no.: 08G0722/11G120). Based on the fraction of HEP in the mixture, the 3-h EC10 is estimated to be > 380 mg/L (worst-case approach; not considering potential mixture toxicity). This value was used for the PNEC calculation.
It can be concluded that inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriately low concentrations.
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