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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Name: Kn 172
Batch no.: Kn-Gi 9328-182
Appearance: white powder
Molecular formula: C13H18N4 x 3HCl
Molecular weight: 339.69 g/mol
Purity: NMR: 96.2%, 0.8% EtOH, 1.4% water
Homogeneity: not stated
Vapour pressure: not stated
Stability: unknown
Solubility H2O: > 1 g/L; EtOH; acetone; CH3CN; DMSO: unknown
Production date: not stated
Expiry date: 10. Jun. 2013
Storage: fridge 2 - 8° C, keep away from light and humidity, keep under Ar!

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
3.65 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
0.83 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Kn 172 is considered very toxic to aquatic invertebrates (Daphnia magna)
Executive summary:

Three experiments were performed under flow-through conditions. As the test item wasn't

stable under test conditions and in the samples which were taken for analytical determination,

the results of the analytical determination in the first and the second experiment were

considered as insufficient. Therefore a third experiment was performed.

The experiments were performed using seven treatments containing 0.046 - 4.6 mg/L

(nominal concentration). Twenty daphnia per concentration were exposed to the test item

for 48 hours in a flow-through test system. After 24 and 48 hours, the immobilised daphnia

were counted.

At the beginning, after 2, 24 and 48 hours, the content of the test item in the test solutions

(inflow and outlet) was determined using HPLC. All samples are measured separately and

directly after sampling and not by using an auto sampler.

The determination of the results was based on the geometric mean of the measured concentrations

in the inflows and the outlets and the arithmetic mean of the four measurements.

In the third experiment, all quality control criteria were met. The 24h-EC50i of potassium

dichromate was tested as positive control. The value was determined as 1.7 mg/L, lying

within the demanded range of 0.6 - 1.7 mg/L.

The following results were determined for the test item Kn 172 (species: Daphnia magna).

24h and 48h NOEC = 0.01 mg/L

24h EC50r = 0.40 mg/L

48h ECsq = 0.16 mg/L

24h EC100r = 3.65 mg/L

48h EC100r = 0.83 mg/L