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EC number: 800-253-4 | CAS number: 1419212-73-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Solubility in organic solvents / fat solubility
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- Stability: thermal, sunlight, metals
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
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- Specific investigations
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- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04-03-2013 to 14-05-2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study according to the guideline under GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Test was performed on water accomodated fraction.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: duplicate samples from all replicates at all concentrations at 0 and 72 h (pooled in main test)
- Sample storage conditions before analysis: at -20 °C - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: stirring for 23 hours, standing 1 hour and transfer via tube with Nescofilm seal by mid depth syphoning using a glass wool plug (WAF)
- Stirring period: recovery as TOC at 100 mg/L after 24 h stirring 2.67 mg/L; 96 h stirring 2.87 mg/L
- Differential loading: 0.10, 0.32, 1.0, 3.2 and 10 mg/L (all WAFs were prepared by dilution of 10 mg/L WAF)
- Controls: untreated
- Evidence of undissolved material: microscopic evaluation of the WAFs did not show any undissolved material - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchnerella subcapitata
- Strain: CCAP 278/4
- Source : Culture Collection of Algea and Protozoa (CCAP), SAMS Research SErvices Ltd., Scottish Marine Institute, Oban, Argyll, Scotland
- Age of inoculum (at test initiation):
- Method of cultivation: under constant aeration and illumination at 21 °C in AAP medium (pH adjusted to 7.5) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 ± 1 °C
- pH:
- at t=0 7.6-7.7
at t=72 7.9-8.2 - Nominal and measured concentrations:
- main test
nominal loading rates: 0.10, 0.32, 1.0, 3.2 and 10 mg/L
measured:
t=0 0.119, 0.103, 0.214, 0.687 and 2.13 mg/L;
t=72measured (TWA): 0.075, 0.067, 0.12, 0.36 and 1.09 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL conical flask with polyurethane foam bug filled with 100 mL
- Aeration: none, sheken at 150 rpm
- Initial cells density: 5E03 cells/mL
- Control end cells density: 5E03 cells/mL (exponential growth observed during the 72 h test period)
- No. of vessels per concentration (replicates): 3 for test substance concentrations
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes AAP
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: continuously light (380-730 nm)
- Light intensity: 7000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Coulter counter at 0, 24, 48 and 72 hours
- Chlorophyll measurement: none
TEST CONCENTRATIONS:
- Range finding study: Loading rates: first 10 and 100 mg/L ; second 0.01, 0.1, and 1.0 mg/L
- Test concentrations:Loading rate: 0.10, 0.32, 1.0, 3.2 and 10 mg/L
- Results used to determine the conditions for the definitive study: inhibition of growth at 1.0 mg/L and above - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 3.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 2.8-3.5 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 1.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.12 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 0.36 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- 52% inhibition at this concentration
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): yes at 0.1, 0.32 and 1.0 mg/L no abnormalities; at 3.2 and 10 mg/L no intact cells present - Results with reference substance (positive control):
- EC50 for potassium dichromate for growth rate 1.2 mg/L
- Reported statistics and error estimates:
- Bartlett test for homogeneity of variance
Dunnett test for statistically differences between test groups and controls - Validity criteria fulfilled:
- yes
- Conclusions:
- The ECr50 for algae is 3.1 mg/L as loading rate. The NOELR is 1.0 mg/L as loading rate (0.12 mg/L measured TWA).
- Executive summary:
Algae were exposed to 0, 0.10, 0.32, 1.0, 3.2 and 10 mg/L test substance during 72 hours. Effects on growth rate and biomass were seen at 3.2 and 10 mg/L with no normal cells present at these concentrations. The analyses for accuracy of preparation showed a decrease over the 72 hour test period. The average measured concentration at the NOELR of 1.0 mg/L was 0.12 mg/L.
The ECr50 for algae is 3.1 mg/L as loading rate. The NOEC is considered to be 0.12 mg/L. The ErC50 based on measured concentrations is ca. 0.36 mg/L.
Reference
Loading rate (mg/L) |
Mean Growth Rate (% of controls) |
Mean Biomass inhibition (% of controls) |
0 |
100 |
100 |
0.10 |
102 |
110 |
0.32 |
101 |
105 |
1.0 |
98 |
90 |
3.2 |
48 |
3 |
10 |
<0 |
<0 |
Description of key information
Algae were exposed to 0, 0.10, 0.32, 1.0, 3.2 and 10 mg/L test substance during 72 hours. Effects on growth rate and biomass were seen at 3.2 and 10 mg/L with no normal cells present at these concentrations. The analyses for accuracy of preparation showed a decrease over the 72 hour test period. The average measured concentration at the NOELR of 1.0 mg/L was 0.12 mg/L.
The ELr50 for algae is 3.1 mg/L as loading rate. The NOEC is considered to be 0.12 mg/L. The ErC50 based on measured concentrations is ca. 0.36 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.36 mg/L
- EC10 or NOEC for freshwater algae:
- 0.12 mg/L
Additional information
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