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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 - 14 Jun 2012
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions: The study is not performed under GLP conditions. No test substance monitoring is performed.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
no
Remarks:
but performed according to ISO 17025
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test solutions are prepared in accordance with the OECD monography n°23. Aqueous fish test medium is saturated with the substance to be tested by dynamic contact setting with a known test substance amount. The preparation consists of 68 h of gentle agitation by magnetic stirring to obtain a vortex of about 1/3 (or less under some conditions) of the height between the top and the bottom of the flask, followed by 4 h of phase separation.The aqueous phase, containing the soluble substances is taken to perform the test. The test solution is identified to its loading rate (massic proportion of substance to be tested/ test medium mass introduced into the diphasic reactor) while operating the Water accomodated fraction (WAF). The implemented loaded ratio is : 0.10/1000.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebra fish
- Source: Bassler Biofish, stationstraat 130, 2235 Westmerbeek
- Length at study initiation: The fish involved in the test had a total length of 25 - 30 mm
- Feeding during test: none

ACCLIMATION
- Acclimation period: The fish have been acclimatized for at least 7 days.
- Acclimation conditions: same as test
- Type and amount of food: not stated
- Feeding frequency: not stated
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
200 - 250 mg/L as CaCO3
Test temperature:
23 ± 1 °C
pH:
6.0 - 8.5
Dissolved oxygen:
7.7 - 8.7 mg/L (> 60% saturation)
Nominal and measured concentrations:
Nominal: 100 mg/L loading rate WAF
Details on test conditions:
TEST SYSTEM
- Test vessel: tank
- Aeration: The aeration was performed by gently blowing bubbles of ambient air into the test vessels.
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.9 - 1.0 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD guideline 203
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH of the control and treatments was measured daily.

OTHER TEST CONDITIONS
- Photoperiod: 16 h photoperiod

EFFECT PARAMETERS MEASURED: Mortality was recorded after 24, 48, 72 and 96 h test duration.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
(WAF)
Basis for effect:
mortality
Details on results:
The fish did not show any abnormal behaviour in the experimental conditions and no external apparent impact was detected. No mortaly was observed in the control and the treatment during the test period of 96 h.

Description of key information

No toxic effects up to the limit of water solubility (< 0.15 mg/L) for Danio rerio (OECD 203) 

Key value for chemical safety assessment

Additional information

One study investigating the short-term toxicity of Hexanoic acid, 2-ethyl-, C16-18 alkyl esters (CAS No. 90411-68-0) to freshwater fish is available. The non-GLP study was conducted under static conditions according to OECD guideline 203 using Danio rerio as test organism (Bouillon, 2012). A 100 mg/L loading rate WAF was tested in a limit test. No mortality was observed in the treatment and the control throughout the test period of 96 h. Hence, a 96 h-LL50 is determined to be > 100 mg/L based on the nominal test concentration.