Pentapotassium bis(peroxymonosulphate) bis(sulphate)

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-11-13 - 2007-01-10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

not indicated

Vehicle:

no

Details on preparation and application of test substrate:

not indicated

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm (Eisenia foetida)
- Source: Vickers Farms, Orlando, Florida
- Age at test initiation: > 2 months, < 12 months
- Weight at test initiation: 300 – 600 mg

ACCLIMATION
- Acclimation period: 48 h
- Acclimation conditions: 18 to 22 °C

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Post exposure observation period:

not indicated

Test temperature:

20°C±2°C

pH:

5.9 – 6.3

Moisture:

30 - 35%

Details on test conditions:

TEST SYSTEM
- Test container (material, size): gass jars of approx. 1.8 L volume
- Amount of soil or substrate: 0.75 kg/container
- No. of organisms per container: 10
- No. of replicates per concentration: 4

SOURCE AND PROPERTIES OF SUBSTRATE
- Composition of artificial substrate: 10% sphagnum, 20% kaolin clay (kaolinite content above 30%), 70% industrial sand (> 50% of the particles between 50 and 200 µm), Calcium carbonate

OTHER TEST CONDITIONS
- Photoperiod: continuous
- Light intensity: 401 – 541 lux

EFFECT PARAMETERS MEASURED: Mortality, biomass
Earthworms were assessed for mortality and behavioural effects after 7 and 14 days of exposure and earthworm body weights were assessed at day 0 and day 14.

VEHICLE CONTROL PERFORMED: no

TEST CONCENTRATIONS

- Range finding study:
- Test concentrations: 0 (control), 0.10, 1.0, 10, 100 and 1000 mg/kg KMPS triple salt/kg dry soil
- Results used to determine the conditions for the definitive study: After seven days of incubation, there was no mortality in the control through the highest treatment level.

Nominal and measured concentrations:

Nominal levels of test concentrations: 65, 130, 250, 500, 1000 mg KMPS/mg dry soil

Reference substance (positive control):

yes

Remarks:

2-Chloroacetamide

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Details are given under "Remarks on results including tables and figures"

Results with reference substance (positive control):

14-d LC50 > 1000 mg/kg dry soil

Reported statistics and error estimates:

No LC50 value could be calculated due to lack of mortality.

Effects on biomass and mortality

Test item concentration (mg/kg soil)	Mean weight change per treatment (mg)	% weight change	Mortality (%)
Control	-682	-15	0
65	-549	-14	0
130	-614	-14	0
250	-563	-13	0
500	-618	-14	0
1000	-597	-14	0

 

Effect data

-	14 d [mg/kg soil]	95 % c.l.
LC0	1000	-
LC50	>1000	-
LC100	not applicable	-

based on nominal concentrations

Validity criteria fulfilled:

yes

Remarks:

(Mortality of control animals < 10% )

Conclusions:

The study and the conclusions which are drawn from it fulfill the quality criteria (validity, reliability, repeatability). The study was performed according to OECD guideline 207. Control mortality was <10%. The validity criterion was met. The LC50 for earthworms was determined to be > 1000 mg/kg soil.

Executive summary:

Materials and methods

The acute toxicity of KMPS triple salt to Eisenia foetida was determined in a dose-response 14-day test according to the OECD guideline 207.

 

Results and discussion

After 14 days of exposure to treated artificial soil the LC50 for earthworms was determined to be >1000 mg KMPS triple salt/mg soil. No effects on biomass or behaviour were evident.

LC0       = 1000 mg/kg soil

LC50      > 1000 mg/kg soil

Description of key information

The toxicity to soil macroorganisms of the test item was tested in an acute toxicity test to earthworm. The test was carried out in accordance with the OECD Guideline 207 (Earthworm, Acute Toxicity Tests).

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw

Additional information

Shot-term toxicity to invertebrates

The acute toxicity of KMPS triple salt to Eisenia foetida was determined in a dose-response 14-day test according to the OECD guideline 207. After 14 days of exposure to treated artificial soil the LC50 for earthworms was determined to be >1000 mg KMPS triple salt/mg soil. No effects on biomass or behaviour were evident.

LC0 = 1000 mg/kg soil

LC50 > 1000 mg/kg soil

 

Long term toxicity to invertebrates

In accordance to section 9.4.4 of Annex X, long-term toxicity to invertebrates, does not need to be conducted if direct and indirect exposure of the soil compartment is unlikely. An acute toxicity test in Eisenia fetida was done with KMPS triple salt. Up to a test concentration of 1000 mg/L no toxic effect could be observed (LC50 > 1000 mg KMPS triple salt/ L). This result shows that KMPS triple salt has a very low acute toxicity to earthworms (IUCLID section 6.3.1). As there are no other indications of concern for the terrestrial compartment, further long-term testing is considered to be scientifically not justified.