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EC number: 808-279-8 | CAS number: 88938-51-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
No adverse effects noted at the limit of solubility
Key value for chemical safety assessment
Additional information
The toxicity of Disperse Blue ANT to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201, Commission Regulation (EC) No, 761/2009, C.3 (2009) and EPA Guideline 712-C-006: OCSPP 850.4500 (2012).The aim of the study was to assess the effects on biomass integrals, growth rate and yield over a period of 96 hours.
The study was conducted under static conditions with an initial cell density of 10010 cells/mL. The saturated solution and further four dilution levels were tested in a geometrical series with a dilution factor of 2:6.25 - 12.5 - 25.0 - 50.0-100 % of the saturated solution, corresponding to the nominal loading rates 0.313 - 0.625 - 1.25 - 2.50 - 5.00 mg/L. Four replicates were tested for each test itemloading rateand eight replicates for the control.
The environmental conditionswere within the acceptable limits.
No recovery phase was performed, because no loading rate was inhibited by more than 50 %.
The concentrations of the test item Disperse Blue ANT in the saturated solution and the controlwere analytically verified via HPLC-UV at the start of exposure. The recovery rates of the test item at the start were below the limit of quantification.
All effect values were based on the nominal loading ratesof the test item Disperse Blue ANT.
The results are summarized in the following table:
NOEL, LOEL, EL-Values of Disperse Blue ANT
|
Inhibition of Biomass Integrals Nominaltest item loading rates [mg/L] |
|
0 - 72 hours |
0 - 96 hours |
|
NOEL |
5.00 |
5.00 |
LOEL |
> 5.00 |
> 5.00 |
EbL10 |
> 5.00 |
> 5.00 |
EbL20 |
> 5.00 |
> 5.00 |
EbL50 |
> 5.00 |
> 5.00 |
|
Inhibition of Growth Rate Nominaltest item loading rates [mg/L] |
|
NOEL |
5.00 |
5.00 |
LOEL |
> 5.00 |
> 5.00 |
ErL10 |
> 5.00 |
> 5.00 |
ErL20 |
> 5.00 |
> 5.00 |
ErL50 |
> 5.00 |
> 5.00 |
|
Inhibition of Yield Nominaltest item loading rates [mg/L] |
|
NOEL |
5.00 |
5.00 |
LOEL |
> 5.00 |
> 5.00 |
EyL10 |
> 5.00 |
> 5.00 |
EyL20 |
> 5.00 |
> 5.00 |
EyL50 |
> 5.00 |
> 5.00 |
In this study,Disperse Blue ANTwas found not to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata with the following effect values (nominal test item loading rates): The EL50-values for inhibition of biomass (EbL50), growth rate (ErL50) and yield (EyL50) after 72 and 96 hours were > 5.00 mg/L. The NOEL-values for inhibition of biomass, growth rate and yield after 72 and 96 hours were 5.00 mg/L.
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