Sodium 5-N-butylbenzotriazole

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 - 18 December 1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

EEC Directive 67/548 Annex V C.1 as published ín 84/449/EEC

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 5-N-butylbenzotriazole

EC Number:

404-450-2

EC Name:

Sodium 5-N-butylbenzotriazole

Cas Number:

118685-34-0

Molecular formula:

CCCCc1ccc2c(c1)nnn2[Na]

IUPAC Name:

sodium 5-butyl-1H-1,2,3-benzotriazol-1-ide

Test material form:

solid - liquid: aqueous solution

Details on test material:

Identification: Butyl benzotriazole, sodium salt (Cobratec 435 - 37% aqueous solution)
Physical state: brown liquid
Analytical purity: 37%
Storage in original container at room temperature in darkness
Method of preparation: direct dispertion in water
Stability of test concentration: verified by chemical analysis. water sample taken from control, 0.7, 1.2, 3.7, 12 and 37 mg a.i./l at 0 and 48 hours and sent to the HRC Department of Analytical Chemistry for analysis by HPLC

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: In original container at room temperature in darkness

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: Sub-samples of the test solutions, following dilution with distilled water where necessary were analysed by high performance liquid chromatography.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Westacre Trout Farm, Norfolk
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 6.8 cm (SD = 0.4 cm)
- Weight at study initiation (mean and range, SD): 4.36 g (SD = 0.64 g)
- Method of breeding:
- Maintenance of the brood fish:

ACCLIMATION
- Acclimation period: 30 October 1989 - 27 November 1989
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food during acclimation: Commercial trout pellets
- Feeding frequency during acclimation: daily. Discontinued 24 h prior to test
- Health during acclimation (any mortality observed): Mortalities: <1% in 7 days prior to the test.
- Medication: None
- Temperature: 14 °C
- Dissolved oxygen: >= 10.1 mgO2/l



FEEDING DURING TEST
- Food type:
- Amount:
- Frequency:

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness = 350 mg/l as CaCO3. This value is slightly higher than the recommended range but is not considered to have had any significant effects on the results of the test.

Test temperature:

13°C

pH:

Concentration mg a.i./l 0 hours 24 hours 48 hours 72 hours 96 hours

pH pH pH pH pH
Control 7.1 7.1 7.1 7.2 7.2
0.67 7.1 7.2 7.2 7.2 7.1
1.2 7.1 7.2 7.2 7.2 7.2
2.1 7.1 7.2 7.2 7.2 7.2
3.7 7.1 7.3 7.3 7.2 7.2
6.7 7.1 7.4 - - -

Dissolved oxygen:

Concentration mg a.i./l 0 hours 24 hours 48 hours 72 hours 96 hours

mgO2/l mgO2/l mgO2/l mgO2/l mgO2/l
Control 9.9 10.1 10.0 10.1 10.0
0.67 9.9 9.8 9.9 10.0 10.0
1.2 9.9 9.8 9.9 10.0 10.0
2.1 9.9 9.8 9.9 10.0 10.0
3.7 9.9 9.8 9.8 10.0 10.0
6.7 9.9 9.8 - - -

Salinity:

see Appendix 3 test report

Conductivity:

see Appendix 3 test report

Nominal and measured concentrations:

37% solution: 1.8, 3.2, 5.6, 10 and 18 mg/l
Active ingredient: 0.67, 1.2, 2.1, 3.7 and 6.7 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass Aquaria holding 40 l of test media
- Aeration: via narrow bore glass tubes
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water, dechlorinated by the addition of sodium thiosulphate. Total hardness = 350 mg/l as CaCO2. Thisvalue is slightly higher than the recommended range but is not considered to have had any significant effects on the results of the test.

OTHER TEST CONDITIONS

- Photoperiod: 16 h light : 8h dark
- Light intensity:

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.8 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:
- Observations on body length and weight:
- Other biological observations: Marked reactions to exposure (other than death) were loss of equiligrium, swimming at surface, coughing and moribundity.
- Mortality of control: No
- Other adverse effects control:No
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No

Any other information on results incl. tables

Sublethal observations / clinical signs:

Concentration mg a.i./l	Abnormality	3 Hours	6 Hours	24 Hours	48 Hours	72 Hours	96 Hours
Control	No abnormalities detected
0.67	No abnormalities detected
1.2	No abnormalities detected
2.1	Swimming at surface	2/10	3/10	2/10
3.7	Coughing Loss of equilibrium Moribund	5/10 8/10	5/10 8/10	5/10 10/10	5/10 10/10	5/5	All fish dead
6.7	Moribund	1/1	All fish dead

Applicant's summary and conclusion

Conclusions:

Highest test concentration resulting in 0% mortality = 2.1 mg/l. Lowest test concentration resulting in 100% mortality = 3.7 mg/l.
The "no-observed effect concentration" is considered to be 1.2 mg/l.

Executive summary:

The acute toxicity (96 h LC50) of Butyl benzotriazole, sodium salt was tested on Rainbow trout in semi-static test conditions according to OECD Guideline No. 203 and EEC directive 67/548 Annex V C. l as published in 84/449/EEC.

Time (h)	LC50 (mg/l)	95% confidence limits mg/l
3	5.1	4.4 - 6.0
6	5.0	3.7 - 6.7*
24	5.0	3.7 - 6.7*
48	5.0	3.7 - 6.7*
72	3.8	3.1 - 4.7
96	2.8	2.1 - 3.7*

* Concentrations resulting in 0% mortality and 100 % mortality respectively.

Highest test concentration resulting in 0% mortality = 2.1 mg/l. Lowest test concentration resulting in 100% mortality = 3.7 mg/l.

The "no-observed effect concentration" is considered to be 1.2 mg/l.